M cell targeting with Aleuria aurantia lectin as a novel approach for oral allergen immunotherapy Franziska Roth-Walter, PhD, Isabella Schöll, PhD, Eva.

Slides:

Advertisements

Similar presentations

Secretory IgA induces antigen-independent eosinophil survival and cytokine production without inducing effector functions Kathleen R. Bartemes, BA, Kate.

Advertisements

Detection of novel occupational wood allergens in locust wood dust (Robinia pseudoacacia L.) Sabine Kespohl, PhD, Rolf Merget, MD, Axel Overlack, MD,

Kiyoshi Yamazaki, MD, Joseph A. Murray, MD, Hirohito Kita, MD

Repeated low-dose intradermal allergen injection suppresses allergen-induced cutaneous late responses Giuseppina Rotiroti, MD, Mohamed Shamji, PhD, Stephen.

Birch pollen–related food allergy: Clinical aspects and the role of allergen-specific IgE and IgG4 antibodies Marija Geroldinger-Simic, MD, Thomas Zelniker,

Cor a 1–reactive T cells and IgE are predominantly cross-reactive to Bet v 1 in patients with birch pollen–associated food allergy to hazelnut Claudia.

Relevance of specific IgE antibody titer to the prevalence, severity, and persistence of asthma among 19-year-olds in northern Sweden Matthew S. Perzanowski,

Mapping of conformational IgE epitopes on Phl p 5a by using mimotopes from a phage display library Brigitte Hantusch, MSc, Sigurd Krieger, TA, Eva Untersmayr,

High-titer IgE antibody specific for pollen allergens in northern California is associated with both wheezing and total serum IgE Elizabeth A. Erwin,

Antacid medication inhibits digestion of dietary proteins and causes food allergy Eva Untersmayr, MD, Isabella Schöll, MSc, Ines Swoboda, PhD, Waltraud.

IgE-mediated systemic anaphylaxis and impaired tolerance to food antigens in mice with enhanced IL-4 receptor signaling Clinton B. Mathias, PhD, Suejy.

Advances in food allergy in 2015

Designing hypoallergenic derivatives for allergy treatment by means of in silico mutation and screening Theresa Thalhamer, PhD, Heidi Dobias, MSc, Tatjana.

Santa Jeremy Ono, BA, PhD, Mark B. Abelson, MD

Maternal allergy increases susceptibility to offspring allergy in association with TH2- biased epigenetic alterations in a mouse model of peanut allergy

Giant ragweed specific immunotherapy is not effective in a proportion of patients sensitized to short ragweed: Analysis of the allergenic differences.

Differential cytokine induction by the human skin–associated autoallergen thioredoxin in sensitized patients with atopic dermatitis and healthy control.

Relevance of specific IgE antibody titer to the prevalence, severity, and persistence of asthma among 19-year-olds in northern Sweden Matthew S. Perzanowski,

Cor a 1–reactive T cells and IgE are predominantly cross-reactive to Bet v 1 in patients with birch pollen–associated food allergy to hazelnut Claudia.

Development and characterization of a recombinant, hypoallergenic, peptide-based vaccine for grass pollen allergy Margarete Focke-Tejkl, PhD, Milena.

Blocking antibodies induced by immunization with a hypoallergenic parvalbumin mutant reduce allergic symptoms in a mouse model of fish allergy Raphaela.

Mapping of conformational IgE epitopes on Phl p 5a by using mimotopes from a phage display library Brigitte Hantusch, MSc, Sigurd Krieger, TA, Eva Untersmayr,

Allergen-dependent solubilization of IL-13 receptor α2 reveals a novel mechanism to regulate allergy Michael O. Daines, MD, Weiguo Chen, PhD, Yasuhiro.

Epicutaneous sensitization results in IgE-dependent intestinal mast cell expansion and food-induced anaphylaxis Lisa M. Bartnikas, MD, Michael F. Gurish,

Birch pollen immunotherapy results in long-term loss of Bet v 1–specific TH2 responses, transient TR1 activation, and synthesis of IgE-blocking antibodies

Is 9 more than 2 also in allergic airway inflammation?

Kiyoshi Yamazaki, MD, Joseph A. Murray, MD, Hirohito Kita, MD

Incomplete digestion of codfish represents a risk factor for anaphylaxis in patients with allergy Eva Untersmayr, MD, Helle Vestergaard, Hans-Jørgen.

Long-term tolerance after allergen immunotherapy is accompanied by selective persistence of blocking antibodies Louisa K. James, PhD, Mohamed H. Shamji,

Antibody conjugates bispecific for intercellular adhesion molecule 1 and allergen prevent migration of allergens through respiratory epithelial cell layers

Investigation of peanut oral immunotherapy with CpG/peanut nanoparticles in a murine model of peanut allergy Kamal D. Srivastava, PhD, Alyssa Siefert,

Oral immunotherapy induces local protective mechanisms in the gastrointestinal mucosa Stephanie A. Leonard, MD, Gustavo Martos, PhD, Wei Wang, MD, Anna.

Peanut-induced intestinal allergy is mediated through a mast cell–IgE–FcεRI–IL-13 pathway Meiqin Wang, MD, PhD, Katsuyuki Takeda, MD, PhD, Yoshiki Shiraishi,

Protein unfolding strongly modulates the allergenicity and immunogenicity of Pru p 3, the major peach allergen Masako Toda, PhD, Gerald Reese, PhD, Gabriele.

A novel allergen-specific therapy with CD40-silenced B cells and dendritic cells Motohiko Suzuki, MD, PhD, Makoto Yokota, MD, PhD, Yoshihisa Nakamura,

Phillip Lieberman, MD, Michael Tankersley, MD

Downregulation of a disintegrin and metalloproteinase 33 by IFN-γ in human airway smooth muscle cells Isao Ito, MD, PhD, Johanne D. Laporte, PhD, Pierre.

Time for a paradigm shift in asthma treatment: From relieving bronchospasm to controlling systemic inflammation Leif Bjermer, MD Journal of Allergy.

Oral administration of a synthetic agonist of Toll-like receptor 9 potently modulates peanut-induced allergy in mice Fu-Gang Zhu, PhD, Ekambar R. Kandimalla,

Allergen-specific immunotherapy with recombinant grass pollen allergens Marek Jutel, MD, Lothar Jaeger, MD, Roland Suck, PhD, Hanns Meyer, Dipl Math,

Peanut T-cell epitope discovery: Ara h 1

Liam O’Mahony, PhD, Mübeccel Akdis, MD, PhD, Cezmi A. Akdis, MD

Autophagy: Nobel Prize 2016 and allergy and asthma research

Allergen-specific sublingual immunotherapy is dose and duration dependent in a murine allergic rhinitis model Soichi Tofukuji, PhD, Kazufumi Katayama,

Genetic susceptibility to food allergy is linked to differential TH2-TH1 responses in C3H/HeJ and BALB/c mice Vivian Morafo, PhD*, Kamal Srivastava,

A major allergen gene-fusion protein for potential usage in allergen-specific immunotherapy Fatimah Kussebi, MD, Fariba Karamloo, PhD, Claudio Rhyner,

Association between specific timothy grass antigens and changes in TH1- and TH2-cell responses following specific immunotherapy Véronique Schulten, PhD,

Luisa Lunardon, MD, Aimee S. Payne, MD, PhD

Sara Paveglio, PhD, MS, Erin Bennett, MS, Kelly L. Hawley, PhD, Adam P

Harald Renz, MD, Chaya Brodie, PhD, Katherine Bradley, BS, Donald Y. M

Birch pollen–related food allergy: Clinical aspects and the role of allergen-specific IgE and IgG4 antibodies Marija Geroldinger-Simic, MD, Thomas Zelniker,

Eric B. Brandt, PhD, Ariel Munitz, PhD, Tatyana Orekov, MS, Melissa K

The steroidogenic enzyme Cyp11a1 is essential for development of peanut-induced intestinal anaphylaxis Meiqin Wang, MD, PhD, Julita Ramirez, DVM, PhD,

Liping Xie, MD, John T. Schroeder, PhD, Jacqueline M

Xiu-Min Li, MD, LaVerne Brown, PhD

In vivo effects of glucocorticoids on IgE production

Pedro Gamboa, MD, PhD, Rosa Sanchez-Monge, PhD, M

Thunderstorm-related asthma attacks

Grass pollen immunotherapy: IL-10 induction and suppression of late responses precedes IgG4 inhibitory antibody activity James N. Francis, PhD, Louisa.

Stefan Schülke, PhD, Stefan Vieths, PhD

Eric B. Brandt, PhD, Melissa K. Mingler, MS, Michelle D

Ovalbumin-specific IgE modulates ovalbumin-specific T-cell response after repetitive oral antigen administration Nemuko Omata, MD, Yusei Ohshima, MD,

Innate immune defects correlate with failure of antibody responses to H1N1/09 vaccine in HIV-infected patients Suresh Pallikkuth, PhD, Sudheesh Pilakka.

Repeated low-dose intradermal allergen injection suppresses allergen-induced cutaneous late responses Giuseppina Rotiroti, MD, Mohamed Shamji, PhD, Stephen.

Placental transfer of allergen-specific IgG but not IgE from a specific immunotherapy– treated mother Sabine Flicker, PhD, Katharina Marth, MD, Heinz.

Soybean isoflavones regulate dendritic cell function and suppress allergic sensitization to peanut Madhan Masilamani, PhD, John Wei, BA, Shiven Bhatt,

IgE sensitization profiles toward green and gold kiwifruits differ among patients allergic to kiwifruit from 3 European countries Merima Bublin, MSc,

The effects of gastric digestion on codfish allergenicity

Natural history of cow’s milk allergy

Perturbations of natural killer cell regulatory functions in respiratory allergic diseases Francesca Scordamaglia, MD, Mirna Balsamo, PhD, Antonio Scordamaglia,

Presentation transcript:

M cell targeting with Aleuria aurantia lectin as a novel approach for oral allergen immunotherapy Franziska Roth-Walter, PhD, Isabella Schöll, PhD, Eva Untersmayr, MD, Renate Fuchs, PhD, George Boltz-Nitulescu, PhD, Andrea Weissenböck, PhD, Otto Scheiner, PhD, Franz Gabor, PhD, Erika Jensen-Jarolim, MD Journal of Allergy and Clinical Immunology Volume 114, Issue 6, Pages 1362-1368 (December 2004) DOI: 10.1016/j.jaci.2004.08.010 Copyright © 2004 American Academy of Allergy, Asthma and Immunology Terms and Conditions

Fig 1 A, Adhesion of bare microspheres (1), AAL microspheres (2), or WGA microspheres (3) to Caco2 intestinal epithelial cells. Caco2 cells were incubated with the different types of fluorescein-cadaverine–labeled microspheres. Plasma membranes were localized with rabbit antialkaline phosphatase antibody and Alexa568 conjugated antirabbit IgG. Nuclei were stained with Hoechst dye. B, AAL microparticles (black bars) and WGA (gray bars) bound substantially better to a Caco2 monolayer than bare microspheres (white bars). The intestinal epithelial cell line Caco2 was grown in monolayers and incubated at 4°C with different microsphere preparations containing FITC-cadaverin. Cells with bound microspheres were dissolved and mean fluorescence (n=3) detected at 485/535 nm. rFU, Relative fluorescence units. Journal of Allergy and Clinical Immunology 2004 114, 1362-1368DOI: (10.1016/j.jaci.2004.08.010) Copyright © 2004 American Academy of Allergy, Asthma and Immunology Terms and Conditions

Fig 2 Birch pollen–sensitized mice (n=8 per group) were treated orally with BP-loaded microparticles functionalized with AAL (black columns) or WGA (gray columns) or without functionalization (white columns). Sera before and after treatment were analyzed for BP-specific IgG1 titer, IgE, and IgA titers (μg/mL serum) in ELISA. ∗P < .05. Journal of Allergy and Clinical Immunology 2004 114, 1362-1368DOI: (10.1016/j.jaci.2004.08.010) Copyright © 2004 American Academy of Allergy, Asthma and Immunology Terms and Conditions

Fig 3 Mice (n=8 per group) were sensitized to BP and afterward treated orally with BP-loaded microparticles. Splenocytes proliferated in all groups specifically to BP, but not with the control allergens of grass pollen (GP). Feedings of these mice were performed with BP-loaded AAL microparticles (dark gray columns), WGA microparticles (gray columns), or uncoated microparticles (white columns). cpm, Counts per minute. Journal of Allergy and Clinical Immunology 2004 114, 1362-1368DOI: (10.1016/j.jaci.2004.08.010) Copyright © 2004 American Academy of Allergy, Asthma and Immunology Terms and Conditions

Fig 4 Supernatants of splenocytes of BP-sensitized mice (n=8 per group), which were treated orally with BP-loaded microparticles, were analyzed for cytokine contents. WGA-coated and AAL-coated microparticles produced significantly more IL-10 and IL-4 than feedings with bare, BP-loaded microparticles (MSBP). For IFN-γ, only AAL microspheres (AALMS) produced significant elevation of this cytokine. WGAMS, WGA microspheres. ∗P < .05. Journal of Allergy and Clinical Immunology 2004 114, 1362-1368DOI: (10.1016/j.jaci.2004.08.010) Copyright © 2004 American Academy of Allergy, Asthma and Immunology Terms and Conditions