I. Gurkan, A. Ranganathan, X. Yang, W. E. Horton, M. Todman, J

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Presentation transcript:

Modification of osteoarthritis in the guinea pig with pulsed low-intensity ultrasound treatment I. Gurkan, A. Ranganathan, X. Yang, W.E. Horton, M. Todman, J. Huckle, N. Pleshko, R.G. Spencer Osteoarthritis and Cartilage Volume 18, Issue 5, Pages 724-733 (May 2010) DOI: 10.1016/j.joca.2010.01.006 Copyright © 2010 Terms and Conditions

Fig. 1 Group E2/T4, medial compartment. Thionin stain. A. Left side, PLIUS treated. Medial tibial plateau: intact surface with mild loss of matrix staining in the superficial region. Medial femoral condyle: Intact surface with uniform matrix staining. B. Right side, control. Medial tibial plateau: extensive surface fibrillation with fissuring to the deep zone and superficial loss of matrix staining. Medial femoral condyle: intact surface with moderate loss of matrix staining. Osteoarthritis and Cartilage 2010 18, 724-733DOI: (10.1016/j.joca.2010.01.006) Copyright © 2010 Terms and Conditions

Fig. 2 Group E2/T4, lateral compartment. Thionin stain. A. Left side, PLIUS treated. Tibial plateau: intact surface, no loss of staining. Femoral condyle: intact surface, no loss of staining. B. Right side, control. Tibial plateau: extensive surface irregularities with mild loss of matrix staining. Femoral condyle: intact surface, no loss of staining. C and D. Expansion of indicated regions of Panels A and B. Osteoarthritis and Cartilage 2010 18, 724-733DOI: (10.1016/j.joca.2010.01.006) Copyright © 2010 Terms and Conditions

Fig. 3 Group E2/T10, medial compartment. Thionin stain. A. Left side, PLIUS treated. Tibial plateau: intact surface with moderate staining loss. Femoral condyle: intact surface with minimal matrix staining loss. B. Right side, control. Tibial plateau: small surface irregularity. Femoral condyle: fissuring to the middle zone and moderate loss of matrix staining. Osteoarthritis and Cartilage 2010 18, 724-733DOI: (10.1016/j.joca.2010.01.006) Copyright © 2010 Terms and Conditions

Fig. 4 Group E12/T6, Lateral compartment. Thionin stain. A. Left side, PLIUS treated. Lateral tibial plateau: intact surface and staining. Lateral femoral condyle: intact surface and staining. B. Right side, control. Lateral tibial plateau: fissuring to middle zone with focal mild matrix staining loss. Lateral femoral condyle: intact surface and uniform staining. C and D. Expansion of indicated regions of Panels A and B. Osteoarthritis and Cartilage 2010 18, 724-733DOI: (10.1016/j.joca.2010.01.006) Copyright © 2010 Terms and Conditions

Fig. 5 Percent of cells showing positive staining for IL-1ra, MMP-3, and MMP-13 in group E2/T4. On average, PLIUS treatment resulted in a trend for a smaller percentage of cells to express MMP-3, and a statistically significant reduction in the percentage of cells expressing MMP-13. Osteoarthritis and Cartilage 2010 18, 724-733DOI: (10.1016/j.joca.2010.01.006) Copyright © 2010 Terms and Conditions

Fig. 6 Representative results of immunostaining for TGF-β1 in the femoral condyles of group E2/T4. A. Expression of TGF-β1 is seen throughout the articular surface of non-treated joint. 4× fixed objective. B. Expansion of region of Panel A. Association of TGF-β1 expression with chondrocytes in clusters. 10× fixed objective. C. Representative region of a different untreated limb, again indicating widespread TGF-β1 expression. 4× fixed objective. D. Dramatic reduction of TGF-β1 expression seen in articular cartilage exposed to PLIUS treatment; shown is the treated joint contralateral to the control joint shown in Fig. 6(A). 10× fixed objective. In all animals, PLIUS-treated articular cartilage displayed minimal expression of TGF-β1. Osteoarthritis and Cartilage 2010 18, 724-733DOI: (10.1016/j.joca.2010.01.006) Copyright © 2010 Terms and Conditions