Wheat lipid transfer protein is a major allergen associated with baker's asthma Arantxa Palacin, PhD, Santiago Quirce, MD, PhD, Alicia Armentia, MD, PhD,

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Wheat lipid transfer protein is a major allergen associated with baker's asthma Arantxa Palacin, PhD, Santiago Quirce, MD, PhD, Alicia Armentia, MD, PhD, Mar Fernández-Nieto, MD, Luis F. Pacios, PhD, Teresa Asensio, MD, Joaquin Sastre, MD, PhD, Araceli Diaz-Perales, PhD, Gabriel Salcedo, PhD Journal of Allergy and Clinical Immunology Volume 120, Issue 5, Pages 1132-1138 (November 2007) DOI: 10.1016/j.jaci.2007.07.008 Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Protein staining (A) and immunodetection with anti-peach LTP Pru p 3 polyclonal antibodies (B) or IgE of a serum pool from patients with baker's asthma (C) or a negative control serum pool (see the Methods section; D) of the following SDS-PAGE–separated samples: extract from whole seed (Wh), bran (Br), and flour (Fl) of bread wheat and purified allergens Tri a 14 (Tr14) and Pru p 3 (Pr3). M, Molecular weight markers. Journal of Allergy and Clinical Immunology 2007 120, 1132-1138DOI: (10.1016/j.jaci.2007.07.008) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Alignment of the amino acid sequences of wheat Tri a 14 (deduced from the cDNA clone Ta Ltp 9.1b; accession no. AJ784902) and peach Pru p 3.20 The experimental N-terminal sequence determined for Tri a 14 is underlined. Sequential IgE epitopes previously located in the Pru p 3 sequence32 are boxed. Journal of Allergy and Clinical Immunology 2007 120, 1132-1138DOI: (10.1016/j.jaci.2007.07.008) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 ELISA-inhibition assays with purified Tri a 14 (A) and Pru p 3 (B) in the solid phase and as inhibitors. Only those sera shown in Fig 3, A, that display significant specific IgE levels to Pru p 3 (Table I) are included in Fig 3, B. Means (n = 2) and SDs (bars) are represented. Journal of Allergy and Clinical Immunology 2007 120, 1132-1138DOI: (10.1016/j.jaci.2007.07.008) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 4 Epitopes previously defined in Pru p 332(top) shown also for Tri a 14 (bottom): E1, residues 11 to 20 (red); E2, residues 31 to 40 (green); and E3, residues 71 to 80 (yellow). A, Ribbon diagram; B, molecular surface; C, Isocontours −1.0 (red) and +1.0 (blue) of the PB potential superimposed on the surfaces in Fig 4, B. Journal of Allergy and Clinical Immunology 2007 120, 1132-1138DOI: (10.1016/j.jaci.2007.07.008) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions