Follicular viability and morphology of sheep ovaries after exposure to cryoprotectant and cryopreservation with different freezing protocols Banu Demirci,

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Presentation transcript:

Follicular viability and morphology of sheep ovaries after exposure to cryoprotectant and cryopreservation with different freezing protocols Banu Demirci, Jacqueline Lornage, M.D., Ph.D., Bruno Salle, M.D., Ph.D., Lucien Frappart, M.D., Ph.D., Michel Franck, Jean François Guerin, M.D., Ph.D. Fertility and Sterility Volume 75, Issue 4, Pages 754-762 (April 2001) DOI: 10.1016/S0015-0282(00)01787-8

Figure 1 The morphology of primordial follicles (arrows) after incubation in 2 M of dimethyl sulfoxide (original magnification, ×400). Demirci. Cryopreservation of sheep ovarian tissue. Fertil Steril 2001. Fertility and Sterility 2001 75, 754-762DOI: (10.1016/S0015-0282(00)01787-8)

Figure 2 Results of trypan blue staining. (A), Dead follicles; (B), live follicles. Arrows = 50 μm. Demirci. Cryopreservation of sheep ovarian tissue. Fertil Steril 2001. Fertility and Sterility 2001 75, 754-762DOI: (10.1016/S0015-0282(00)01787-8)

Figure 3 Follicular mortality after exposure of the ovarian tissue to dimethyl sulfoxide (shaded bars) or propylene glycol (white bars). ∗ = Significantly different from the dimethyl sulfoxide control group (rDMSO=−0.99). ∗∗ = Significantly different from the propylene glycol control group (rPROH=−0.98). Demirci. Cryopreservation of sheep ovarian tissue. Fertil Steril 2001. Fertility and Sterility 2001 75, 754-762DOI: (10.1016/S0015-0282(00)01787-8)

Figure 4 Results of cooling without (A) and with (B) semiautomatic seeding. The temperature of tubes containing ovarian tissue is plotted as a function of time. …. = reference; ________ = medium; - - - - = N2 tank. Demirci. Cryopreservation of sheep ovarian tissue. Fertil Steril 2001. Fertility and Sterility 2001 75, 754-762DOI: (10.1016/S0015-0282(00)01787-8)

Figure 5 Primordial follicle morphology after freezing-thawing. Semiautomatic seeding protocol with 2 M dimethyl sulfoxide (original magnification, ×400). The long arrow indicates intact oocytes; the short arrow indicates an oocyte with pycnotic nucleus. Demirci. Cryopreservation of sheep ovarian tissue. Fertil Steril 2001. Fertility and Sterility 2001 75, 754-762DOI: (10.1016/S0015-0282(00)01787-8)

Figure 6 Follicular mortality after freezing-thawing of the ovarian tissue by using freezing without semiautomatic seeding (shaded bars) or freezing with semiautomatic seeding (white bars). ∗P<0.01 compared with propylene glycol freezing protocols with seeding. ∗∗P<0.02 compared with dimethyl sulfoxide freezing protocols with seeding. ∗∗∗P<0.01 compared with propylene glycol freezing protocols without seeding. Demirci. Cryopreservation of sheep ovarian tissue. Fertil Steril 2001. Fertility and Sterility 2001 75, 754-762DOI: (10.1016/S0015-0282(00)01787-8)