HRI depletion elevates γ-globin in primary erythroid CD34+ cells

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Fig. 3 HRI depletion elevates γ-globin in primary erythroid CD34+ cells. HRI depletion elevates γ-globin in primary erythroid CD34+ cells. (A) Representative HbF flow cytometry on day 15 of CD34+ erythroid differentiation. SC, sickle cell. (B) Summary of HbF flow cytometry experiments. Error bars represent SEM from three independent donors (*P < 0.05, **P < 0.01 from unpaired Student t tests). (C) γ-Globin mRNA as a fraction of γ-globin plus β-globin by RT-qPCR on day 13. Error bars represent SEM from three independent donors (*P < 0.05, **P < 0.01 from unpaired Student t tests). (D) RNA-seq for CD34+ cells on day 13. r is the Pearson correlation coefficient. Data were obtained from one patient donor. (E) HPLC analysis of samples from cells expressing annotated shRNAs at day 15 of differentiation. HbA, hemoglobin A (adult form). Error bars represent SD from two independent donors. (F) Images of sickle cell patient–derived erythroid cultures. Arrowheads mark cells with sickle-like morphology. The bar graph summarizes blind counts of sickle-shaped cells in three fields from one patient with SCD. Jeremy D. Grevet et al. Science 2018;361:285-290 Copyright © 2018 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works