CD11b-mediated migratory property of peripheral blood B cells

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Presentation transcript:

CD11b-mediated migratory property of peripheral blood B cells Kazushige Kawai, MD, Nelson H. Tsuno, MD, Mika Matsuhashi, Joji Kitayama, MD, Takuya Osada, MD, Jun Yamada, MD, Takeshi Tsuchiya, MD, Satomi Yoneyama, MD, Toshiaki Watanabe, MD, Koki Takahashi, MD, Hirokazu Nagawa, MD Journal of Allergy and Clinical Immunology Volume 116, Issue 1, Pages 192-197 (July 2005) DOI: 10.1016/j.jaci.2005.03.021 Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions

Fig 1 A subset of peripheral blood B cells expresses CD11b. A, Flow-cytometric analysis of PBMC. X-axis represents CD19, and Y-axis, the CD11b expression. The representative dot-plot from 12 independent samples is presented. B, The percentage of CD11b+ B cells. Journal of Allergy and Clinical Immunology 2005 116, 192-197DOI: (10.1016/j.jaci.2005.03.021) Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions

Fig 2 Expression of cell surface molecules. Purified B cells were stained with FITC-mAbs and PE-CD11b mAb, and the expression of cell surface molecules was investigated on the CD11b− (upper) and CD11b+ (lower) populations. In each figure, the bold line represents the negative control, and the solid line, the expression of the respective cell surface markers. Journal of Allergy and Clinical Immunology 2005 116, 192-197DOI: (10.1016/j.jaci.2005.03.021) Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions

Fig 3 CD11b expression of cB1, cB2, and cB3 B-cell subpopulations. A, Purified B cells were stained with FITC–anti-CD27, PE–anti-IgD, and PE-Cy5–anti-CD11b mAbs. The percentage of cB1 (filled bar), cB2 (open bar), and cB3 (shaded bar) subpopulations among purified B cells (left), CD11b− B cells (middle), and CD11b+ B cells (right) is shown. Data are presented as means ± SDs from 3 independent experiments using samples from the same donor. ∗Statistically significant difference from the result of total purified B cells. B, The CD11b expressions of cB1 (blue line), cB2 (red line), and cB3 (green line) subpopulations are presented. Filled black line represents the negative control. Journal of Allergy and Clinical Immunology 2005 116, 192-197DOI: (10.1016/j.jaci.2005.03.021) Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions

Fig 4 Migration assay. A, Purified B cells were allowed to migrate in a transwell assay system. Purified B cells as well as migrated cells were stained with anti-CD11b mAb, and the percentage of CD11b+ cells was evaluated by flow cytometry. B, Alternatively, cells were stained with anti-CD27 and anti-IgD mAbs, and the percentage of cB1 (filled bar), cB2 (open bar), and cB3 (shaded bar) subpopulations was evaluated. Data are presented as means ± SDs from triplicate experiments. ∗Statistically significant difference from the result of purified B cells. Journal of Allergy and Clinical Immunology 2005 116, 192-197DOI: (10.1016/j.jaci.2005.03.021) Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions

Fig 5 Effect of CD11b blocking with the specific mAb on the migratory activity of B cells. Purified B cells were allowed to migrate in the absence or presence of chemokines in the lower chamber and anti-CD19 or anti-CD11b mAb in the upper chamber. Data are presented as means ± SDs from triplicate experiments. ∗Statistically significant difference compared with the negative control. Journal of Allergy and Clinical Immunology 2005 116, 192-197DOI: (10.1016/j.jaci.2005.03.021) Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions

Fig 6 Expression of chemokine receptors. Purified B cells were stained with FITC-mAbs and PE-CD11b mAb, and the expression of chemokine receptors on CD11b− (upper) and CD11b+ (lower) subpopulations was evaluated. In each figure, the bold line represents negative control, and the solid line, the expression of CXCR4 and CXCR5. Journal of Allergy and Clinical Immunology 2005 116, 192-197DOI: (10.1016/j.jaci.2005.03.021) Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions