iGAMA knockdown parasites are defective in host cell attachment.

Slides:

Advertisements

Similar presentations

Reverse Genetic Screen in Toxoplasma gondii

Advertisements

From: CXCL10 Is Required to Maintain T-Cell Populations and to Control Parasite Replication during Chronic Ocular Toxoplasmosis Invest. Ophthalmol. Vis.

From: Migration of Toxoplasma gondii –Infected Dendritic Cells across Human Retinal Vascular Endothelium Invest. Ophthalmol. Vis. Sci ;53(11):

TIA-1 Self-Multimerization, Phase Separation, and Recruitment into Stress Granules Are Dynamically Regulated by Zn2+ Joseph B. Rayman, Kevin A. Karl,

Volume 3, Issue 2, Pages (February 2008)

Janice M Dobrowolski, L.David Sibley Cell

by Leila M. Lopes Bezerra, and Scott G. Filler

DHEA(S) and Allo activated transcription factors NF-κB and CREB in serum-deprived PC12 cells. DHEA(S) and Allo activated transcription factors NF-κB and.

Volume 15, Issue 5, Pages (May 2016)

Volume 13, Issue 3, Pages (March 2013)

Michelle Woldemar Carr, Ronen Alon, Timothy A Springer Immunity

Ang II induces translocation of α-ENaC toward the apical membrane.

Volume 125, Issue 1, Pages (July 2003)

Effects of length-altering chemical treatments are more apparent after F-L synchronization. Effects of length-altering chemical treatments are more apparent.

(A) Growth curves of strains at 30°C in SPP medium or SPP supplemented with 15 μM paclitaxel or 10 μM oryzalin. (A) Growth curves of strains at 30°C in.

by Silvia Mele, Stephen Devereux, Andrea G

Kala Subramanian, Tobias Meyer Cell

Mutants impaired in N-acetylglucosamine transport and catabolism display morphological and pH neutralization defects within macrophages. Mutants impaired.

TgGAMA is a secreted micronemal protein capable of binding host cells.

Commensal bacteria reduce the stability of influenza A virus.

Aldolase Is Essential for Energy Production and Bridging Adhesin-Actin Cytoskeletal Interactions during Parasite Invasion of Host Cells G. Lucas Starnes,

Wnt5A Activates the Calpain-Mediated Cleavage of Filamin A

ERMES impacts on the exposure of pathogen-associated molecular patterns during hyphal growth. ERMES impacts on the exposure of pathogen-associated molecular.

MMV is a novel antifungal agent.

Adapted viruses replicate in mismatched miR-122-expressing cells.

Identification of apicomplexan-specific putative invasion proteins.

Heparin enhances cell retention within a biofilm but does not promote attachment. Heparin enhances cell retention within a biofilm but does not promote.

Effect of small TbTim knockdown on cell growth.

Malaria Sporozoites Traverse Host Cells within Transient Vacuoles

C. jejuni flaC mutants induce increased cell activation in human and chicken cells. C. jejuni flaC mutants induce increased cell activation in human and.

Volume 107, Issue 4, Pages (August 2014)

Thermal and pH stability of influenza viruses.

Volume 8, Issue 4, Pages (October 2010)

Volume 14, Issue 7, Pages (February 2016)

Δcarp mutants have a growth phenotype in vitro and in vivo.

Volume 13, Issue 2, Pages (October 2015)

TIA-1 Self-Multimerization, Phase Separation, and Recruitment into Stress Granules Are Dynamically Regulated by Zn2+ Joseph B. Rayman, Kevin A. Karl,

Localization of PBANKA_ and PBANKA_ during blood and liver stage development. Localization of PBANKA_ and PBANKA_ during blood.

Translocation of a Vibrio cholerae Type VI Secretion Effector Requires Bacterial Endocytosis by Host Cells Amy T. Ma, Steven McAuley, Stefan Pukatzki,

Establishment of a TgGAMA conditional-knockdown strain.

Effects of neomycin on early stages of the CVB3-Nancy replication cycle. Effects of neomycin on early stages of the CVB3-Nancy replication cycle. (A) Cell.

SsC6TF1 (Sscle04g036970) regulates sclerotial development and virulence on pea. SsC6TF1 (Sscle04g036970) regulates sclerotial development and virulence.

Global Analysis of Palmitoylated Proteins in Toxoplasma gondii

Readthrough GPx1 Ter–Neptune proteins colocalize with UPF1 under cytochalasin D treatment. 6CFSMEo- cells transfected with plasmids encoding YFP–UPF1 (green),

Pharmacological inhibition of phosphodiesterases can repair phenotypic defects in the TgATPaseP-GC mutant. Pharmacological inhibition of phosphodiesterases.

Arginine-to-lysine mutations conferring TRIM22 restriction and lysine-to-arginine mutations causing loss of TRIM22 restriction. Arginine-to-lysine mutations.

E. coli CFT073 and E. coli MG1655 persistence.

Defective MEK-Erk1/2 kinase activation in RIAM-depleted cells accounts for their impaired FA disassembly. Defective MEK-Erk1/2 kinase activation in RIAM-depleted.

Domain organization of GAMA proteins and proteolytic processing of TgGAMA. Domain organization of GAMA proteins and proteolytic processing of TgGAMA. (A)

Effects of neomycin on CVB3-Nancy replication kinetics.

Optimization of a MYC degradation screen.

SAM transport by Gap4 in S. cerevisiae cells.

LACK/LACKDDE L. major show robust nascent global protein synthesis at 27 and 35°C. LACK/LACKDDE L. major show robust nascent global protein synthesis at.

Distribution of signaling molecules in a section of P

Effective TcdB cell interactions correlate with endocytosis.

Generation of GAMA knockout (KO) by CRISPR-CAS9.

RNF2 promotes TGFβ signaling.

Overexpression of AP2IV-3 enhances tissue cyst formation.

Low pH represses growth of cells with the hyphal morphology and promotes growth as pseudohyphae. Low pH represses growth of cells with the hyphal morphology.

Effects of HDAC2 inactivation on the invasive potential of human gastric cancer cells. Effects of HDAC2 inactivation on the invasive potential of human.

The effects of HDAC2 knockdown on cell-cycle proteins.

FBXL19-AS1 knockdown inhibits proliferation, migration and invasion, and reduces the expression levels of angiogenesis related proteins in lung cancer.

Osteoactivin expression is required for the invasive phenotype of in vivo selected bone metastatic 4T1 breast cancer cells. Osteoactivin expression is.

GlcN-induced knockdown of PfHsp70x does not affect intraerythrocytic growth. GlcN-induced knockdown of PfHsp70x does not affect intraerythrocytic growth.

WLBU2 disrupts P. aeruginosa biofilms during RSV coinfection of bronchial epithelial cells. WLBU2 disrupts P. aeruginosa biofilms during RSV coinfection.

Characterization of SFV-specific small RNAs bound by Piwi4 and Ago2 in Aag2 cells. Characterization of SFV-specific small RNAs bound by Piwi4 and Ago2.

GBM cells infected in vitro with HCMV divide less often than uninfected cells do. GBM cells infected in vitro with HCMV divide less often than uninfected.

GBM 112 and GBM 144 cells infected in vitro with HCMV resist growth inhibition by temozolomide. GBM 112 and GBM 144 cells infected in vitro with HCMV resist.

Translocation of a Vibrio cholerae Type VI Secretion Effector Requires Bacterial Endocytosis by Host Cells Amy T. Ma, Steven McAuley, Stefan Pukatzki,

PfHsp70x knockout does not inhibit export of PfEMP1 to the host cell.

Presentation transcript:

iGAMA knockdown parasites are defective in host cell attachment. iGAMA knockdown parasites are defective in host cell attachment. (A) Red-green invasion assay of tachyzoites after 20 min of incubation with HFF cells. (B) Attachment of cytochalasin D-treated tachyzoites to HFF cells. Parasites were preloaded with calcein green AM and then incubated with cytochalasin D and allowed to attach to host cells for 15 min with cytochalasin D. BAPTA-AM-treated Δku80:Tati parasites are a negative control for attachment. (C) Timed invasion assay. Shown are the results of a red-green invasion assay of tachyzoites after 0.5, 1, 2, 4, or 8 h of incubation with HFF cells. (D) Induced-egress assay. Egress from vacuoles grown for 28 h in chamber slides was induced with the calcium ionophore A23187, and occupied versus unoccupied vacuoles were enumerated. (E) Modes of gliding motility. Parental and iGAMA+ATc tachyzoites with different types of gliding motility were enumerated by video microscopy. Data are the average ± the standard error of the mean of at least three independent experiments for all graphs. *, P < 0.05; ns, not significant. My-Hang Huynh, and Vern B. Carruthers mSphere 2016; doi:10.1128/mSphere.00012-16