Biogal Galed Labs. Experience - established in 1986. Kibbutz owned company A leader in veterinary diagnostic kits. Serologic and direct evaluation of veterinary diseases. Customers > 30 countries.

Scandinavian Countries Austria + Central Europe Biogal’s Markets Scandinavian Countries Netherlands Korea UK France Austria + Central Europe USA* Spain India Japan* Germany Italy Ecuador Israel Philippines Thailand Brazil* Chile Australia

Connections with Research Institutes Worldwide

ImmunoComb® Antibody Test Kits

ImmunoComb®: Creating New Opportunities in Poultry Health

The ImmunoComb®: A Diagnostic Tool The ImmunoComb® Based on solid-phase ELISA principle. Provides serological results; measuring antibody levels in blood or serum to specific disease (e.g., viruses, mycoplasma, rickettsia).

ImmunoComb® –Product Range Companion Animals Canine Feline Avian Livestock Bovine Additional CombScan Custom-made Kits Poultry Turkey Chicken

ImmunoComb® Key Products – Poultry Poultry Mycoplasma (Mg, Ms, Mm) (3 in 1) Poultry IBD-ND-IB (3 in 1)

ImmunoComb® Poultry Antibody Test Kit - Contents

ImmunoComb® Process 1) Obtain blood/serum/disc/ egg yolk from animal/s 2) Deposit sample/s into well/s A in developing plate

ImmunoComb® Process (Continued) 3) Insert Comb into well/s A. Incubate for a specified time 4) Transfer the Comb from well/s A to well/s F in a timed sequence, according to specific kit. 5) Let comb dry and Read results. The color intensity of spots on comb corresponds to antibody level

Read Results 1 2 3 4 IBD ND IB Tooth 1: Negative IBD (S1); High positive ND (S5); Positive IB (S4). Tooth 2: Positive IBD (S3); Low positive ND (S2); Negative IB (S0). Tooth 3: Positive control (S3). Tooth 4: Negative control (S0).

Result interpretation – 3 types Provides qualitative results Visual interpretation semi-quantitative results CombScale (histogram) quantitative results CombScan (Densitometry)

Applications of the ImmunoComb® Epidemic Prevention Case Study in Poland Problem: Early detection of disease in the field. Solution: Routine use of the ImmunoComb® assessing flock’s health. Results: Early discovery of IBD (Gumboro) in unvaccinated broiler flock. Decreased mortality. Clear quantitative results documented in the field using the CombScan.

Applications of the ImmunoComb® Maintaining Breeder Flock Health Case Study in Israel Problem: Government regulation demand breeder flocks be free of Mycoplasma. Solution: Bi-monthly use of ImmnunoComb® Mg-Ms kit assessing flock’s health. Results: Improved Mycoplasma surveillance in breeder flocks. Early detection prevent financial losses. Using the ImmunoComb® allows for confidentiality & early decision making.

Applications of the ImmunoComb® Vaccination Monitoring Case Study in Mexico Problem: Monitoring vaccine levels in the field. Solution: Routine use of the ImmunoComb® assessing flock’s health. Results: Increased confidence of poultry farmer concerning the efficacy of vaccine. Increased productivity due to shorter testing time. Clear quantitative results documented in the field using the CombScan.

Biogal’s ImmunoComb® Advantages Farm Animals ELISA 1 week?!, $$$ to end user ImmunoComb 30 minutes

ImmunoComb® comparison with ELISA Reliable and accurate quantitative results at qualitative speed. Industry standard for reliable and accurate quantitative results Simultaneous testing of multiple disease agents on one Individual ELISA test kits are required for each disease agent Portable - can be performed in field or on-site Requires expensive analytical equipment in a laboratory setting Flexible - specimen can be taken from egg yolk, blood on paper disc, blood or serum Specimen from blood or serum only

ImmunoComb® comparison with ELISA Continued…. Ideal for rapid analysis of small or individual flock samples. Large numbers of samples are needed for one 'test-run'. ImmunoComb® contains pre- diluted reagents and materials for faster sample processing Slow sample processing and cumbersome dilution steps Faster result turn-around time. Longer result turn-around time. User friendly, easily learned technique. Must be performed by a trained lab technician.

For Technical Support Please Contact: E-mail: info@biogal.co.il Thank You! For Technical Support Please Contact: Biogal – Galed Labs. Tel: 972-4-9898605, E-mail: info@biogal.co.il www.biogal.co.il