Analysis of follicular fluid hormone concentrations and granulosa cell mRNA levels for the inhibin-activin-follistatin system: relation to oocyte and.

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Analysis of follicular fluid hormone concentrations and granulosa cell mRNA levels for the inhibin-activin-follistatin system: relation to oocyte and embryo characteristics Toshihiro Fujiwara, M.D., Ph.D., Geralyn Lambert-Messerlian, Ph.D., Yisrael Sidis, Ph.D., Lucy Leykin, Ph.D., Keith Isaacson, M.D., Thomas Toth, M.D., Alan Schneyer, Ph.D. Fertility and Sterility Volume 74, Issue 2, Pages 348-355 (August 2000) DOI: 10.1016/S0015-0282(00)00652-X

Figure 1 Relation of hormone concentration to oocyte maturational stage. Follicular fluid hormone concentrations were compared with characteristics of oocytes from the same follicles. (A), Free follistatin concentrations were significantly lower in hFF from follicles containing GV oocytes compared with follicles containing MII oocytes. (B), Progesterone concentrations were significantly lower in hFF from follicles with GV oocytes compared with MI or MII oocytes. The horizontal lines designate the 10th, 50th, and 90th percentiles for each group. The difference between the groups labeled “a” and “b” is statistically significant (P<.01). Fujiwara. Follicular fluid hormone concentrations. Fertil Steril 2000. Fertility and Sterility 2000 74, 348-355DOI: (10.1016/S0015-0282(00)00652-X)

Figure 2 Relation of granulosa-cell inhibin α-subunit mRNA and oocyte and embryo characteristics. The relation between gene expression in granulosa cells and characteristics of the oocyte contained in the same follicles were analyzed. Of all targets examined, only inhibin α-subunit mRNA levels were associated with oocyte and embryo characteristics; these levels were significantly higher in healthy, mature oocytes. In contrast, oocytes producing the healthiest embryos came from follicles with significantly lower α-subunit expression compared with abnormal embryos; this suggests that although some α-subunit expression is associated with normal oocyte maturation, excessive α-subunit is associated with abnormal embryo development. Levels of mRNA were expressed as ratios to β-actin for each target, log transformed, and corrected for age on the basis of linear regressions; they are shown as multiples of the age-specific median (MoM). Horizontal lines indicate 10th, 50th, and 90th percentiles. The groups labeled “a” and “b” differed significantly. (A), Levels of inhibin α-subunit mRNA were higher in follicles containing MI or MII oocytes compared with GV oocytes (P=.0004, analysis of variance). (B), Levels of inhibin α-subunit mRNA were higher in follicles containing high-quality, grade 1 oocytes (P=.001, analysis of variance). (C), Levels of inhibin α-subunit mRNA were higher in follicles containing fertilized oocytes (P=.008, analysis of variance). (D), Levels of inhibin α-subunit mRNA were lower in follicles that produced normal embryos (P=.008, analysis of variance). Fujiwara. Follicular fluid hormone concentrations. Fertil Steril 2000. Fertility and Sterility 2000 74, 348-355DOI: (10.1016/S0015-0282(00)00652-X)