A. baumannii stimulates the autophagy.

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【 Supplement methods 】 Real-Time Reverse Transcription-PCR Total RNA was isolated from HRGEc using the RNeasy® Mini Kit. cDNA was synthesized using Super-Script®

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Presentation transcript:

A. baumannii stimulates the autophagy. A. baumannii stimulates the autophagy. (A) Expression of human autophagy genes after A. baumannii infection. Total RNA was isolated from A549 cells infected with A. baumannii ATCC 17978 and uninfected cells. cDNAs were synthesized by reverse transcription of the total RNA. A real-time PCR analysis was performed by using the Stratagene Mx3005p system. Samples were normalized to beta-2-microglobulin. Human autophagy gene expression after infection is represented by the heat map. Results are representative of two independent experiments. (B) Western blot analysis of LC3B in A549 cells infected with A. baumannii ATCC 17978 for 2 h. The blots were part of the same internally controlled experiment in Fig. 3C. Results are representative of three independent experiments. The solid white line separates the spliced portions between control and infected cells. Raquel Parra-Millán et al. mSphere 2018; doi:10.1128/mSphere.00106-18