Neto2 is upregulated in older DRG cultures and ERK activation is required for Neto2 upregulation in adult DRG neurons. a, Representative Western blot of Neto2 expression in young wild-type DRG cultures at 4 and 24 h. b, Densitometry quantification of Neto2 ... Neto2 is upregulated in older DRG cultures and ERK activation is required for Neto2 upregulation in adult DRG neurons. a, Representative Western blot of Neto2 expression in young wild-type DRG cultures at 4 and 24 h. b, Densitometry quantification of Neto2 expression normalized to actin and represented as the percentage of P5 culture at 24 h. c, Representative Western blot of Neto2 expression in adult wild-type DRG cultures at 4 and 24 h in untreated cultures and at 24 h in cultures treated with the ERK1/2 inhibitor U0126 (50 μm), the PI3K inhibitor wortmannin (10 nm), and the Akt inhibitor triciribine (20 μm). d, Densitometry quantification of Neto2 expression normalized to actin and represented as percentage of control culture at 24 h. Error bars indicate SEM. *p < 0.05; ***p < 0.001. con, Untreated control cultures; U0, cultures treated with U0126; wor, cultures treated with wortmannin; tric, cultures treated with triciribine. Claire G. Vernon, and Geoffrey T. Swanson J. Neurosci. 2017;37:3352-3363 ©2017 by Society for Neuroscience