Volume 12, Issue 23, Pages (December 2002)

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Volume 12, Issue 23, Pages 2002-2013 (December 2002) Experimental RNomics  Claudia Marker, Anja Zemann, Tanja Terhörst, Martin Kiefmann, James P. Kastenmayer, Pamela Green, Jean-Pierre Bachellerie, Jürgen Brosius, Alexander Hüttenhofer  Current Biology  Volume 12, Issue 23, Pages 2002-2013 (December 2002) DOI: 10.1016/S0960-9822(02)01304-0

Figure 1 Expression Analysis of Novel snmRNAs A selection of expressed candidates for novel snmRNAs (Class I and II) in A. thaliana, as deduced by Northern blot analysis including two developmental growth phases of A. thaliana, the seedling (I) or the adult plant (IV). Sizes of RNAs, as estimated by comparison with an internal RNA marker, are indicated on the right. Exposure times of autoradiograms are shown below each blot (d: days; h: hours; min: minutes). Current Biology 2002 12, 2002-2013DOI: (10.1016/S0960-9822(02)01304-0)

Figure 2 Novel snmRNAs in A. thaliana A schematic overview and classification of 140 candidates for small non-messenger RNAs in A. thaliana. Current Biology 2002 12, 2002-2013DOI: (10.1016/S0960-9822(02)01304-0)

Figure 3 Tissue-Specific Expression of snmRNAs from A. thaliana Northern blot analysis showing tissue-specific expression of Class II snmRNA candidates. The clone number is indicated on the left of each Northern blot, and the estimated size of the snmRNA is indicated on the right. The four tissues used in Northern blot analysis are R (root), S (stem), L (leaf), and F (flower). For clone Ath-241, which exhibits two bands on a Northern blot (with sizes of 162 nt and 70 nt), only the 162 nt band shows tissue-specific expression. As an internal control, ubiquitous expression of 7SL RNA is indicated at the top of each panel. Current Biology 2002 12, 2002-2013DOI: (10.1016/S0960-9822(02)01304-0)

Figure 4 Mitochondrion- and Chloroplast-Encoded snmRNAs from A. thaliana Location of snmRNAs on the mitochondrial (left) and the chloroplast genome (right), not drawn to scale. The snmRNA on the left, the genes flanking the snmRNA candidate, as well as the distance of the snmRNA 5′ and 3′ ends to flanking genes (in nt) are indicated. Flanking genes are shown by black arrows; the respective snmRNA genes are indicated by red arrows (mitochondrial genome) or green arrows (chloroplast genome). For snmRNAs that overlap the open reading frame of a gene, the number of overlapping nucleotides is indicated by a negative number. Current Biology 2002 12, 2002-2013DOI: (10.1016/S0960-9822(02)01304-0)