Supplemental Figure S1 A B Adults Neonates Adult CD103+ DC: KdM282-90

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Supplemental Figure S1 A B Adults Neonates Adult CD103+ DC: KdM282-90 Day 1 Day 2 Day 3 Day 7 Adult CD103+ DC: 31.6 38.2 34.3 19.4 KdM282-90 16 12.9 8.5 4.4 DbM187-195 Neonatal CD103+ DC: 8.4 21.7 36.4 38.4 KdM282-90 9.5 11.6 9.7 10 DbM187-195

Supplemental Figure S2 A B C D E F G H

Supplemental Figure S3

Supplemental Figure S4 Naive Day 1 Day 2 Day 3

Supplemental Figure S5 neonate adult A B

Supplemental Figure S6 CD103hi CD103lo Peptide pulse 10-6 M 10-8 M No exogenous peptide 87.2 86.4 89.3 89.7 88.5 77 42 5