Volume 6, Issue 2, Pages 585-588 (March 2013) The Rice ‘Nutrition Response and Root Growth’ (NRR) Gene Regulates Heading Date  Yuman Zhang, Ge Zhang, Na Xiao, Lina Wang, Yaping Fu, Zongxiu Sun, Rongxiang Fang, Xiaoying Chen  Molecular Plant  Volume 6, Issue 2, Pages 585-588 (March 2013) DOI: 10.1093/mp/sss157 Copyright © 2013 The Authors. All rights reserved. Terms and Conditions

Figure 1 Subcellular Localization of NRR and the Alteration of the Heading Date Phenotype Exhibited in NRRa Transgenic Rice. (A) Cells bombarded with 35S:NRRa–eGFP showing the prevailed nuclear localization of NRRa. (B, D) The same cells as in (A) and (C), but subjected to plasmolysis. (C) Green fluorescence of GFP in cells bombarded with 35S:NRRb–eGFP was found primarily at the cell membrane. (E) Cells bombarded with a control construct 35S:eGFP, showing green fluorescence throughout the cytoplasm and nucleus. Bars in (A)–(D) = 100 μm, in (E) = 200 μm. (F) The late-flowering phenotype of PNRR:NRRa–eGFP plants grown at Beijing. The PNRR:NRRa–eGFP T0 plant (right) with a high expression level of NRRa displayed an obvious delayed heading date phenotype compared with the vector control plant (left). (G) The late-flowering phenotype of 35S:NRRa plants grown at Hainan. When the wild-type plant (left) at the mature stage developed mature yellow seeds, the 35S:NRRa plant (right) was still at the early heading stage with green leaves. (H) Comparison of the flowering percent in NRR–RNAi transgenic plants grown at Beijing. Flowering time was evaluated by the percentage flowering at different times. NRRab–RNAi and miRNRRa plants showed higher flowering percent at 108–112 d after sowing compared with that of wild-type and vector control plants. (I) The expression levels of NRRa in homozygous (Homo), heterozygous (Hetero), and non-transformed (Non) plants derived from a selected 35S:NRRa line. The transcript levels were determined by real-time RT–PCR; ACTIN was used as an internal control. The vector-transformed plant (vector) was used as a negative control. Values shown are means ± SE of triplicate measurements from a single plant. (J) Comparison of the expression of rice flowering time-related genes, Ehd1, Hd3a, and RFT1, in 35S:NRRa plants by real-time RT–PCR. Four independent lines of 35S:NRRa were identified. The transcription level of Ehd1 was increased compared with that of the wild-type plants and those of Hd3a and RFT1 were decreased in 35S:NRRa plants. Error bars indicate SE (n = 3). * indicates the significant difference at P = 0.05 in SPSS paired-samples t-test. Molecular Plant 2013 6, 585-588DOI: (10.1093/mp/sss157) Copyright © 2013 The Authors. All rights reserved. Terms and Conditions