Fig. 2 HRI depletion elevates γ-globin in HUDEP2 cells.

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Fig. 2 HRI depletion elevates γ-globin in HUDEP2 cells. HRI depletion elevates γ-globin in HUDEP2 cells. (A) Representative HbF FACS for HUDEP2-Cas9 sgRNA pools. (B) Summary of HbF flow cytometry of cells expressing indicated sgRNAs. Data are means ± SD from biological replicates (n = 2). (C) Western blots with antibodies against the indicated proteins in cell pools expressing sgRNAs as indicated. Diff, differentiation. (D) Mass spectrometry for the HRI sgRNA 5 pool from biological replicates (n = 3). Data are log2 mean-normalized protein abundances in cells with HRI sgRNA 5 and nontargeting sgRNA. r is the Pearson correlation coefficient. HBZ, ζ-globin; HBA1/2, α-globins 1 and 2. (E) RNA-seq of cell pools with HRI sgRNA 5. Data are log2 numbers of fragments per kilobase of transcript per million mapped reads (FPKM) averaged from biological replicates (n = 2). Jeremy D. Grevet et al. Science 2018;361:285-290 Copyright © 2018 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works