Expression of adrenomedullin in the human corpus luteum

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Expression of adrenomedullin in the human corpus luteum Kazuko Abe, M.D., Takashi Minegishi, M.D., Yoshito Ibuki, M.D., Masayasu Kojima, Ph.D., Kenji Kangawa, Ph.D. Fertility and Sterility Volume 74, Issue 1, Pages 141-145 (July 2000) DOI: 10.1016/S0015-0282(00)00585-9

Figure 1 Tissue-specific expression of adrenomedullin (AM) transcript. Total RNA (10 μg) extracted from human corpus luteum (1), myometrium uterus (2), placenta (3), and fetus membrane (4) was fractionated on denaturing 2% agarose gels and transferred to nylon membranes. Membranes were hybridized by using the AM cRNA probe, as described in the Materials and Methods section. GAPDH = glyceraldehyde-3-phosphate dehydrogenase. Abe. Adrenomedullin in corpus luteum. Fertil Steril 2000. Fertility and Sterility 2000 74, 141-145DOI: (10.1016/S0015-0282(00)00585-9)

Figure 2 Northern blot analysis of LH/hCG receptor, adrenomedullin (AM) and receptor activity–modifying protein 2 (RAMP2) mRNA in human corpus luteum. (A), Northern blot analysis of LH/hCG receptor, AM, and RAMP2 mRNA in human corpus luteum during the menstrual cycle and early pregnancy. Ten micrograms of total RNA from individual samples were fractionated on an agarose gel, transferred to membranes, and hybridized by using the cRNA probes. These data represent the results of four experiments: follicle (F) (cycle day 14, 20 mm in diameter), mid-luteal phase (L) (cycle day 24), pregnancy at 7 weeks P7w, and pregnancy at 13 weeks (P13w). (B), Autoradiographs of RAMP2, AM, and LH/hCG receptor (5.4 kilobases) mRNA were quantified by using densitometric scanning. The amount of RAMP2 mRNA in the follicle was assumed to be 100% and the amounts of AM and LH/hCG receptor mRNA in the mid-luteal phase were assumed to be 100%. Data were normalized for levels of glyceraldehyde-3-phosphate dehydrogenase mRNA levels in each sample and are expressed relative to the control value (mean±SE; n=3). ∗ Difference from the control value at P<.05. Abe. Adrenomedullin in corpus luteum. Fertil Steril 2000. Fertility and Sterility 2000 74, 141-145DOI: (10.1016/S0015-0282(00)00585-9)