NAPRT expression correlates to sensitivity for NAMPT inhibitors and to methylation of the CpG island of the NAPRT promoter. NAPRT expression correlates.

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NAPRT expression correlates to sensitivity for NAMPT inhibitors and to methylation of the CpG island of the NAPRT promoter. NAPRT expression correlates to sensitivity for NAMPT inhibitors and to methylation of the CpG island of the NAPRT promoter. A, NAMPT and NAPRT are heterogeneously expressed in the different chondrosarcoma cell lines. Expression levels were determined using qRT-PCR analyses. B, Log IC50s for the NAMPT inhibitors FK866 and GMX1778 significantly correlated to NAPRT expression levels. C, NAPRT methylation in a previously published methylation array of 10 chondrosarcoma cell lines. β-Values are visualized using Gene-E (Broad Institute), where red represents high methylation of the NAPRT promoter and green represents low methylation of the NAPRT promoter. D, NAPRT expression significantly correlates to methylation of the CpG island of the NAPRT promoter in chondrosarcoma cell lines. E, Pretreating JJ012 cells for 72 hours with AGI-5198, followed by a 72-hour combined treatment of AGI-5198 and the corresponding NAMPT inhibitor did not influence the dose-dependent decrease in cell viability. Cell viability was determined by a PrestoBlue assay. Elisabeth F.P. Peterse et al. Mol Cancer Res 2017;15:1714-1721 ©2017 by American Association for Cancer Research