Histone H4 Is a Major Component of the Antimicrobial Action of Human Sebocytes Dong-Youn Lee, Chun-Ming Huang, Teruaki Nakatsuji, Diane Thiboutot, Sun-Ah.

Slides:

Advertisements

Similar presentations

Figure S1. Production of recombinant NS1 protein

Advertisements

IL-18 Downregulates Collagen Production in Human Dermal Fibroblasts via the ERK Pathway Hee Jung Kim, Seok Bean Song, Jung Min Choi, Kyung Moon Kim,

Amanda M. Nelson, Kathryn L. Gilliland, Zhaoyuan Cong, Diane M

Fig. 6. Sh-lantibiotics are commonly found on healthy human skin and synergize with a host AMP. Sh-lantibiotics are commonly found on healthy human skin.

Macrophage-Derived Metalloelastase Is Responsible for the Generation of Angiostatin in Lewis Lung Carcinoma Zhongyun Dong, Rakesh Kumar, Xiulan Yang,

Exogenous Smad3 Accelerates Wound Healing in a Rabbit Dermal Ulcer Model Koji Sumiyoshi, Atsuhito Nakao, Yasuhiro Setoguchi, Ko Okumura, Hideoki Ogawa

Anan Abu Ubeid, Longmei Zhao, Ying Wang, Basil M. Hantash

Importance of Tissue Transglutaminase in Repair of Extracellular Matrices and Cell Death of Dermal Fibroblasts After Exposure to a Solarium Ultraviolet.

Human peptidoglycan recognition protein S is an effector of neutrophil-mediated innate immunity by Ju Hyun Cho, Iain P. Fraser, Koichi Fukase, Shoichi.

Overexpression of Laminin-8 in Human Dermal Microvascular Endothelial Cells Promotes Angiogenesis-Related Functions Jie Li, Lisa Zhou, Hoang T. Tran,

Effects of Betulinic Acid Alone and in Combination with Irradiation in Human Melanoma Cells Edgar Selzer, Emilio Pimentel, Volker Wacheck, Werner Schlegel,

Volume 94, Issue 1, Pages (July 1998)

Human Skin is a Steroidogenic Tissue: Steroidogenic Enzymes and Cofactors Are Expressed in Epidermis, Normal Sebocytes, and an Immortalized Sebocyte Cell.

UV Modulation of Subcutaneous Fat Metabolism

A Proteolytic Cascade of Kallikreins in the Stratum Corneum

Functions of Rhomboid Family Protease RHBDL2 and Thrombomodulin in Wound Healing Tsung-Lin Cheng, Yu-Ting Wu, Hung-Yu Lin, Fu-Chih Hsu, Shi-Kai Liu,

Aquaporin 3 Colocates with Phospholipase D2 in Caveolin-Rich Membrane Microdomains and Is Downregulated Upon Keratinocyte Differentiation Xiangjian Zheng,

Activation of TLR2 by a Small Molecule Produced by Staphylococcus epidermidis Increases Antimicrobial Defense against Bacterial Skin Infections Yuping.

Protodynamic Intracellular Acidification by cis-Urocanic Acid Promotes Apoptosis of Melanoma Cells In Vitro and In Vivo Jarmo K. Laihia, Janne P. Kallio,

Indu Mani, Vincent A. Ziboh Journal of Investigative Dermatology

Masamoto Murakami, Takaaki Ohtake, Robert A. Dorschner, Richard L

Cross-Linking of SPINK6 by Transglutaminases Protects from Epidermal Proteases Jan Fischer, Yulia Koblyakova, Ties Latendorf, Zhihong Wu, Ulf Meyer-Hoffert

Component-Resolved Diagnosis (CRD) of Type I Allergy with Recombinant Grass and Tree Pollen Allergens by Skin Testing Susanne Heiss, Rudolf Valenta

RNase 7 Protects Healthy Skin from Staphylococcus aureus Colonization

Molecular Therapy - Methods & Clinical Development

Analysis of Proteins with Caseinolytic Activity in a Human Stratum Corneum Extract Revealed a Yet Unidentified Cysteine Protease and Identified the So-Called.

Histone Acetylation in Keratinocytes Enables Control of the Expression of Cathelicidin and CD14 by 1,25-Dihydroxyvitamin D3 Jürgen Schauber, Yuko Oda,

IGF-1 Induces SREBP-1 Expression and Lipogenesis in SEB-1 Sebocytes via Activation of the Phosphoinositide 3-Kinase/Akt Pathway Terry M. Smith, Kathryn.

Plasminogen-Dependent Matriptase Activation Accelerates Plasmin Generation by Differentiating Primary Human Keratinocytes Ya-Wen Chen, Shi Yin, Ying-Jung.

Staphylococcus aureus Hijacks a Skin Commensal to Intensify Its Virulence: Immunization Targeting β-Hemolysin and CAMP Factor Chih-Wei Lo, Yiu-Kay Lai,

Characterization of Group X Phospholipase A2 as the Major Enzyme Secreted by Human Keratinocytes and its Regulation by the Phorbol Ester TPA Gérard Lambeau,

Anti-Inflammatory Activity of Sertaconazole Nitrate Is Mediated via Activation of a p38– COX-2–PGE2 Pathway Runa Sur, Jeffrey M. Babad, Michelle Garay,

T Cell Stimulating Stratum Corneum Antigens: Characterization by Chromatography and Electrophoresis Indicates Limited Diversity Jonathan M. Hales, Richard.

Antibodies Elicited by Inactivated Propionibacterium acnes-Based Vaccines Exert Protective Immunity and Attenuate the IL-8 Production in Human Sebocytes:

S100A15, an Antimicrobial Protein of the Skin: Regulation by E

Volume 74, Issue 5, Pages (May 1998)

Elastin Peptides Induce Migration and Terminal Differentiation of Cultured Keratinocytes Via 67 kDa Elastin Receptor in Vitro: 67 kDa Elastin Receptor.

Kallikrein Expression and Cathelicidin Processing Are Independently Controlled in Keratinocytes by Calcium, Vitamin D3, and Retinoic Acid Shin Morizane,

SPLA2-X Stimulates Cutaneous Melanocyte Dendricity and Pigmentation Through a Lysophosphatidylcholine-Dependent Mechanism Glynis A. Scott, Stacey E.

Decreased Susceptibility of Staphylococcus aureus Small-Colony Variants toward Human Antimicrobial Peptides Regine Gläser, Karsten Becker, Christof von.

A Role for Ran-GTP and Crm1 in Blocking Re-Replication

The Melanocortin 5 Receptor is Expressed in Human Sebaceous Glands and Rat Preputial Cells Diane Thiboutot, Aruntha Sivarajah, Kathryn Gilliland, Zhaoyuan.

Human β-Defensin-2 Production in Keratinocytes is Regulated by Interleukin-1, Bacteria, and the State of Differentiation Alice Y. Liu, Delphine Destoumieux,

Volume 125, Issue 2, Pages (April 2006)

Clinical Snippets Journal of Investigative Dermatology

Antimicrobial Property of Lauric Acid Against Propionibacterium Acnes: Its Therapeutic Potential for Inflammatory Acne Vulgaris Teruaki Nakatsuji, Mandy.

c-Src Activates Endonuclease-Mediated mRNA Decay

UVB and Proinflammatory Cytokines Synergistically Activate TNF-α Production in Keratinocytes through Enhanced Gene Transcription Muhammad M. Bashir,

Signaling Events During Induction of Plasminogen Activator Inhibitor-1 Expression by Sphingosylphosphorylcholine in Cultured Human Dermal Fibroblasts

Characterization of Keratinocyte Differentiation Induced by Ascorbic Acid: Protein Kinase C Involvement and Vitamin C Homeostasis1 Isabella Savini, Antonello.

Arsenic Induces Tumor Necrosis Factor α Release and Tumor Necrosis Factor Receptor 1 Signaling in T Helper Cell Apoptosis Hsin-Su Yu, Gwo-Shing Chen

IL-18 Downregulates Collagen Production in Human Dermal Fibroblasts via the ERK Pathway Hee Jung Kim, Seok Bean Song, Jung Min Choi, Kyung Moon Kim,

Collagen Synthesis Is Suppressed in Dermal Fibroblasts by the Human Antimicrobial Peptide LL-37 Hyun Jeong Park, Dae Ho Cho, Hee Jung Kim, Jun Young.

Belén López-García, Phillip H. A. Lee, Kenshi Yamasaki, Richard L

Volume 95, Issue 2, Pages (October 1998)

Society for Investigative Dermatology 2010 Meeting Minutes

Md Nasimuzzaman, Danielle Lynn, Johannes CM van der Loo, Punam Malik

Insulin-Like Growth Factor-1 Induces Lipid Production in Human SEB-1 Sebocytes Via Sterol Response Element-Binding Protein-1 Terry M. Smith, Zhaoyuan.

Pimecrolimus Enhances TLR2/6-Induced Expression of Antimicrobial Peptides in Keratinocytes Amanda S. Büchau, Jürgen Schauber, Thomas Hultsch, Anton Stuetz,

Volume 108, Issue 1, Pages (January 2015)

Volume 60, Issue 3, Pages (September 2001)

Ken Inoki, Masakazu Haneda, Shiro Maeda, Daisuke Koya, Ryuichi Kikkawa

Suppression of VEGFR2 Expression in Human Endothelial Cells by Dimethylfumarate Treatment: Evidence for Anti-Angiogenic Action Markus Meissner, Monika.

Production of the Soluble Form of KIT, s-KIT, Abolishes Stem Cell Factor-Induced Melanogenesis in Human Melanocytes Shinya Kasamatsu, Akira Hachiya,

Relationship Between Cell-Associated Matrix Metalloproteinase 9 and Psoriatic Keratinocyte Growth Nathalie Buisson-Legendre, Hervé Emonard, Philippe.

Envoplakin and Periplakin, the Paraneoplastic Pemphigus Antigens, are also Recognized by Pemphigus Foliaceus Autoantibodies Shideh Kazerounian, Mỹ G.

Increased Migration of Murine Keratinocytes Under Hypoxia Is Mediated by Induction of Urokinase Plasminogen Activator Richard J. Daniel, Richard W. Groves,

Fig. 6. Sh-lantibiotics are commonly found on healthy human skin and synergize with a host AMP. Sh-lantibiotics are commonly found on healthy human skin.

Volume 22, Issue 3, Pages (May 2006)

Doxycycline Indirectly Inhibits Proteolytic Activation of Tryptic Kallikrein-Related Peptidases and Activation of Cathelicidin Kimberly N. Kanada, Teruaki.

Presentation transcript:

Histone H4 Is a Major Component of the Antimicrobial Action of Human Sebocytes Dong-Youn Lee, Chun-Ming Huang, Teruaki Nakatsuji, Diane Thiboutot, Sun-Ah Kang, Marc Monestier, Richard L. Gallo Journal of Investigative Dermatology Volume 129, Issue 10, Pages 2489-2496 (October 2009) DOI: 10.1038/jid.2009.106 Copyright © 2009 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Purification of the antimicrobial activity from extracts of cultured sebocytes. (a) RP-HPLC was carried out to separate antimicrobial peptides from acid-soluble extracts of SEB-1 sebocytes. A plot of absorbance at 214nm is indicated on the left y axis and that of the concentration of acetonitrile used for elution on the right y axis. (b) The antimicrobial activity of the HPLC fractions was evaluated against S. aureus ΔmprF by solution-killing assay. Growth of bacterial colonies on agar after incubation with fractions obtained by RP-HPLC separation is shown with limiting dilutions from top to bottom. A single fraction (fraction 13) showed maximal antimicrobial activity. (c) HPLC fractions were subjected to SDS-PAGE and stained with Coomassie's blue. Arrow in the active fraction indicates a unique protein band that was further analyzed by mass spectrometry. Journal of Investigative Dermatology 2009 129, 2489-2496DOI: (10.1038/jid.2009.106) Copyright © 2009 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Identification of histone H4 in antimicrobial fractions purified from cultured sebocytes. (a) Western blot analysis with anti-histone antibody (BWA3, specific for histones H2A and H4) was carried out on HPLC fractions shown in Figure 1. In fraction 13, a single strong band was observed at 11kDa, which corresponded to the expected mass of histone H4. In fraction 12, a single strong band was observed at 13kDa, which corresponded to the expected mass of histone H2A. The lane marked as H4 was loaded with recombinant histone H4 used as a positive control. (b) Western blot analysis with the specific anti-acetyl histone H4 antibody showed a single band at 11kDa only in fraction 13. (c) Acid-soluble extracts of SEB-1 sebocytes and the culture medium containing SEB-1 sebocyte debris was evaluated by western blot with an anti-histone H2A and H4 antibody (BWA-3). Two bands were observed at 11 and 13kDa, which corresponded to histones H4 and H2A. H4, recombinant histone H4, was used as a positive control. Journal of Investigative Dermatology 2009 129, 2489-2496DOI: (10.1038/jid.2009.106) Copyright © 2009 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Recombinant histone H4 kills S. aureus and P. acnes. The antimicrobial activity of recombinant histone H4 was evaluated against S. aureus ΔmprF and P. acnes by the solution-killing assay. Bacteria were incubated for 3hours at 37°C in a 10mM sodium phosphate buffer (pH 6.5) containing 100mM NaCl. Recombinant histone H4 showed significant bactericidal activity against S. aureus ΔmprF at 25μgml-1 and above and against P. acnes at 12.5μgml-1 and above. Data shown are from triplicate determinations (**P<0.01; Student's t-test). Journal of Investigative Dermatology 2009 129, 2489-2496DOI: (10.1038/jid.2009.106) Copyright © 2009 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Recombinant histone H4 enhances the antimicrobial action of free fatty acids. The antimicrobial activity of histone H4 was evaluated in the presence of free fatty acids. Lauric and oleic acids (1.5μgml-1) synergistically exerted antimicrobial effects in combination with histone H4. Lauric acid (60μgml-1) exerted a stronger synergy with histone H4, but oleic acid at this concentration killed all bacteria. Data shown are from triplicate determinations (*P<0.05; Student's t-test). DMSO, dimethyl sulfoxide: LA, lauric acid: OA, oleic acid. Journal of Investigative Dermatology 2009 129, 2489-2496DOI: (10.1038/jid.2009.106) Copyright © 2009 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 5 Neutralization of histone H4 in sebocyte extracts removes antimicrobial activity. BWA-3 neutralizing antibody was used to treat extracts of SEB-1 sebocytes. Addition of BWA-3 (400μgml-1) to the extracts of SEB-1 sebocytes significantly decreased antimicrobial activity against S. aureus ΔmprF compared with an equal concentration of IgG added to separate aliquots, or aliquots to which an equal volume of PBS was added as controls. Data shown are from triplicate determinations (*P<0.05; Student's t-test). Journal of Investigative Dermatology 2009 129, 2489-2496DOI: (10.1038/jid.2009.106) Copyright © 2009 The Society for Investigative Dermatology, Inc Terms and Conditions