Shifting meiotic to mitotic spindle assembly in oocytes disrupts chromosome alignment Spinning disk confocal microscopy images showing spindle region magnifications.

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Presentation transcript:

Shifting meiotic to mitotic spindle assembly in oocytes disrupts chromosome alignment Spinning disk confocal microscopy images showing spindle region magnifications of controls (left panel) and oocytes overexpressing HSET WT (right panel) at NEBD+1h30 and +6h30. All oocytes express GFP‐EB3 (green) and Histone‐RFP (blue). The white asterisk marks a chromosome outside of the metaphase plate. Scale bar 10 μm. Spinning disk confocal microscopy images showing spindle region magnifications of controls (left panel) and oocytes overexpressing HSET WT (right panel) before and after anaphase. Oocytes express GFP‐EB3 (green, left panel) or GFP‐HSET WT (green, right panel) and Histone‐RFP (blue). The white asterisk marks a lagging chromosome in the oocyte before and after anaphase. Scale bar 10 μm. Graph representing the percentage of oocytes with aligned (gray) and not aligned (black) chromosomes before anaphase, quantified for controls (left bar), and oocytes expressing HSET WT (right bar). Statistical significance of differences is assessed with a Fisher test: **P‐value = 0.006. Spinning disk confocal microscopy images showing spindle region magnifications of controls (left panel) and oocytes overexpressing HSET N593K (right panel) before and after anaphase. Oocytes express GFP‐EB3 (green, left panel) or GFP‐HSET N593K (green, right panel) and Histone‐RFP (blue). Scale bar 10 μm. Graph representing the percentage of oocytes with aligned (gray) and not aligned (black) chromosomes before anaphase, quantified for controls (left bar) and HSET N593K oocytes (right bar). Statistical significance of differences is assessed with a Fisher test: not significant (n.s.) P‐value > 0.99. IF THIS IMAGE HAS BEEN PROVIDED BY OR IS OWNED BY A THIRD PARTY, AS INDICATED IN THE CAPTION LINE, THEN FURTHER PERMISSION MAY BE NEEDED BEFORE ANY FURTHER USE. PLEASE CONTACT WILEY'S PERMISSIONS DEPARTMENT ON PERMISSIONS@WILEY.COM OR USE THE RIGHTSLINK SERVICE BY CLICKING ON THE 'REQUEST PERMISSIONS' LINK ACCOMPANYING THIS ARTICLE. WILEY OR AUTHOR OWNED IMAGES MAY BE USED FOR NON-COMMERCIAL PURPOSES, SUBJECT TO PROPER CITATION OF THE ARTICLE, AUTHOR, AND PUBLISHER. EMBO Rep, Volume: 19, Issue: 2, Pages: 368-381, First published: 12 January 2018, DOI: (10.15252/embr.201745225)