The Egr inhibitor construct selectively blocks neurite outgrowth induced by NGF. PC12 cells were transfected with a GFP expression plasmid with or without.

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The Egr inhibitor construct selectively blocks neurite outgrowth induced by NGF. PC12 cells were transfected with a GFP expression plasmid with or without Δ(1-249)Egr3, and cell morphology was monitored by fluorescence microscopy.Top, The typical small, rou... The Egr inhibitor construct selectively blocks neurite outgrowth induced by NGF. PC12 cells were transfected with a GFP expression plasmid with or without Δ(1-249)Egr3, and cell morphology was monitored by fluorescence microscopy.Top, The typical small, round, and flat morphology found in undifferentiated PC12 cells (left; control) is shown. After treatment with NGF (right), cells elaborate one or more neurites. Middle, The Egr inhibitor construct, which does not induce neurite outgrowth (left), blocks neurite outgrowth triggered by NGF [right; NGF + Δ(1-249)Egr3]. Bottom, Neurite outgrowth can also be induced by db-cAMP (left) that is not affected by cotransfection with the Egr inhibitor construct [right; db-cAMP + Δ(1-249)Egr3]. Images shown were obtained 2 d after addition of NGF or db-cAMP. Yechiel Levkovitz et al. J. Neurosci. 2001;21:45-52 ©2001 by Society for Neuroscience