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Estimates are from linear regression with robust ... Estimates are from linear regression with robust standard errors to account for heteroscedasticity. Grey area is the 95% CI for the regression line. Multivariable adjusted was for age, BMI, alcohol intake, systolic blood pressure, intake of beef, plasma CRP as continuous variables and sex, smoking status (never smoker, former smoker and or current smoker), fruit and vegetable intake (high, medium or low intake), income (in six groups), physical activity during leisure time (in four groups), any use of vitamins and diuretics (yes/no) and quintiles of plasma creatinine as categorical variables, as well as lot number or year and month of blood sampling. Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) 2018. Published by Oxford University Press on behalf of the British Society for Rheumatology. All rights reserved. For permissions, please email: journals.permissions@oup.comThis article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model) Rheumatology (Oxford), Volume 57, Issue 10, 23 June 2018, Pages 1769–1776, https://doi.org/10.1093/rheumatology/key171 The content of this slide may be subject to copyright: please see the slide notes for details.

Step 1: Association between plasma vitamin C and plasma urate Step 1: Association between plasma vitamin C and plasma urate. Step 2: Association between ... Step 1: Association between plasma vitamin C and plasma urate. Step 2: Association between SLC23A1 rs33972313 and plasma vitamin C and association between SLC2A9 rs7442295 and plasma urate. Step 3: Association between SLC23A1 rs33972313 and plasma urate and association between SLC2A9 rs7442295 and plasma vitamin C. Step 4: Instrumental variable analysis of genetically determined plasma vitamin C on plasma urate. Dotted line: association may be confounded. Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) 2018. Published by Oxford University Press on behalf of the British Society for Rheumatology. All rights reserved. For permissions, please email: journals.permissions@oup.comThis article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model) Rheumatology (Oxford), Volume 57, Issue 10, 23 June 2018, Pages 1769–1776, https://doi.org/10.1093/rheumatology/key171 The content of this slide may be subject to copyright: please see the slide notes for details.

Mean and 95% ... Mean and 95% CI from linear regression adjusted for sex and age. P for trend is from Cuzick’s non-parametric trend test. P is from Kruskal-Wallis test. Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) 2018. Published by Oxford University Press on behalf of the British Society for Rheumatology. All rights reserved. For permissions, please email: journals.permissions@oup.comThis article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model) Rheumatology (Oxford), Volume 57, Issue 10, 23 June 2018, Pages 1769–1776, https://doi.org/10.1093/rheumatology/key171 The content of this slide may be subject to copyright: please see the slide notes for details.

Fig. 4 Observational and genetic estimates of the association between plasma vitamin C and ... Hyperuricaemia was defined as plasma urate >360µmol/l for both men and women. In the two-sample instrumental variable analysis, the gene-exposure estimate is from a meta-analysis by Timpson et al. [13], and the gene-outcome estimate is from the Copenhagen General Population Study. Multivariable adjusted was for sex, age, smoking status, alcohol intake, fruit and vegetable intake, intake of beef, income, physical activity during leisure time, BMI, systolic blood pressure, any use of vitamins, plasma creatinine and plasma CRP. Genetic estimate was adjusted for sex and age. OR: odds ratio. Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) 2018. Published by Oxford University Press on behalf of the British Society for Rheumatology. All rights reserved. For permissions, please email: journals.permissions@oup.comThis article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model) Rheumatology (Oxford), Volume 57, Issue 10, 23 June 2018, Pages 1769–1776, https://doi.org/10.1093/rheumatology/key171 The content of this slide may be subject to copyright: please see the slide notes for details.