Atrial‐like cardiomyocytes from human pluripotent stem cells are a robust preclinical model for assessing atrial‐selective pharmacology AAP illustrating.

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Atrial‐like cardiomyocytes from human pluripotent stem cells are a robust preclinical model for assessing atrial‐selective pharmacology AAP illustrating the analyzed parameters. BRepresentative APs of day 31 CMs from control (CT) and RA‐treated (RA) groups at 1 Hz. C–ERMP, APAmax and APAplat (C), dV/dtmax (D) and APD20, APD50 and APD90 of CT and RA CMs (E). FPlot showing all measured APAplat values of CT and RA CMs. GRepresentative APs of CT and RA CMs at 0.5–4 Hz. HAverage APAplat at 0.5–4 Hz. Please note that the AP differences in morphology are present at all measured frequencies. Data information: Data are presented as mean ± SEM. *P < 0.05 by unpaired t‐test or Mann–Whitney rank‐sum test for (C–E). In (C), P = 0.238 for RMP; P < 0.001 for APAmax and APAplat. In (D), P = 0.598 for dV/dtmax. In (E), P ≤ 0.001 for APD20; P = 0.009 for APD50; P = 0.04 for APD90. Two‐way repeated measures ANOVA followed by pairwise comparison using the Student–Newman–Keuls test for (H). *P = 0.002, 0.006, 0.003, 0.004 and 0.003, respectively, for comparison of APAplat between CT and RA groups at frequencies of 0.5, 1.0, 2.0, 3.0 and 4.0 Hz. AP = action potential; APAmax = maximum AP amplitude; APAplat = AP plateau amplitude; APD20, APD50 and APD90 = AP duration at 20, 50, and 90% repolarization, respectively; CMs = cardiomyocytes; dV/dtmax = maximum upstroke velocity; RMP = resting membrane potential. IF THIS IMAGE HAS BEEN PROVIDED BY OR IS OWNED BY A THIRD PARTY, AS INDICATED IN THE CAPTION LINE, THEN FURTHER PERMISSION MAY BE NEEDED BEFORE ANY FURTHER USE. PLEASE CONTACT WILEY'S PERMISSIONS DEPARTMENT ON PERMISSIONS@WILEY.COM OR USE THE RIGHTSLINK SERVICE BY CLICKING ON THE 'REQUEST PERMISSIONS' LINK ACCOMPANYING THIS ARTICLE. WILEY OR AUTHOR OWNED IMAGES MAY BE USED FOR NON-COMMERCIAL PURPOSES, SUBJECT TO PROPER CITATION OF THE ARTICLE, AUTHOR, AND PUBLISHER. EMBO Mol Med, Volume: 7, Issue: 4, Pages: 394-410, First published: 19 February 2015, DOI: (10.15252/emmm.201404757)