Fig. 6 Activating IRF8 promotes macrophage migration during the recovery phase after SCI. Activating IRF8 promotes macrophage migration during the recovery.

Slides:

Advertisements

Similar presentations

Volume 5, Issue 2, Pages (August 2015)

Advertisements

CD169+ macrophages mediate Lm translocation to the splenic T cell zones. CD169+ macrophages mediate Lm translocation to the splenic T cell zones. (A) Confocal.

Fig. 4 The extracellular domain of sortilin is sufficient for BDNF/TrkB signaling. The extracellular domain of sortilin is sufficient for BDNF/TrkB signaling.

Fig. 3 Sortilin deletion unmasks NTSR2 signaling.

Fig. 3 The electrical contact of direct-printed and reconfigured liquid metals. The electrical contact of direct-printed and reconfigured liquid metals.

Fig. 1 KCC2 down-regulation is prevented in sortilin-deficient mice.

Fig. 2 Global production, use, and fate of polymer resins, synthetic fibers, and additives (1950 to 2015; in million metric tons). Global production, use,

Fig. 5 Thermal conductivity of n-type ZrCoBi-based half-Heuslers.

Fig. 1 Characterization of Suv420h dKO mice.

Fig. 3 Exercise training enhances neuronal activity in vivo.

Fig. 4 Rotation of nuclei in oocytes in the primordial follicles.

Fig. 2 Exercise training potentiates synaptic transmissions via mTOR activation. Exercise training potentiates synaptic transmissions via mTOR activation.

Fig. 1 Examples of experimental stimuli and behavioral performance.

Fig. 1 NP-free Ch-CNC droplets.

Fig. 2 Some examples of weekly forecasts (the number of the forecasts are reported on Table 1). Some examples of weekly forecasts (the number of the forecasts.

Fig. 6 Comparison of properties of water models.

Fig. 2 IT1t prevents TLR7-mediated inflammation in pDCs.

Fig. 3 Rotation experiment, setup.

Fig. 1 Product lifetime distributions for the eight industrial use sectors plotted as log-normal probability distribution functions (PDF). Product lifetime.

Fig. 1 Reference-fixing experiment, setup.

Fig. 1 Neu1−/− fibroblasts have characteristics of myofibroblasts.

Fig. 3 FcRL1 is passively recruited into B cell immunological synapses upon BCR engagement in primary spleen B cells. FcRL1 is passively recruited into.

Fig. 6 SNP rs is a functional SNP in 22Rv1 cells.

Fig. 5 Mutation of Y281ENV motif in FcRL1 impairs BCR engagement–induced B cell activation in the absence of FcRL1 ligation. Mutation of Y281ENV motif.

Fig. 1 Genes up-regulated in Zic5 KO cells correlate with the increased glycolytic state. Genes up-regulated in Zic5 KO cells correlate with the increased.

Fig. 4 Reconstitution of MCs in KitW-sh/W-sh mice increases IL-10+ Breg cells and suppresses the CHS response. Reconstitution of MCs in KitW-sh/W-sh mice.

Fig. 2 2D QWs of different propagation lengths.

Fig. 7 LPS enhances cochlear TRPV1 expression and GTTR uptake in vivo.

Fig. 6 WPS imaging of different chemical components in living cells.

Fig. 4 Cdc42 enhances the cellular uptake of EVs.

Fig. 5 Simultaneous absence of caspase-3 and -7 is required for significant decrease of caspase-8 and -9 activation in intrinsic apoptosis. Simultaneous.

Fig. 3 IRF8 is required for macrophage migration toward the epicenter in the injured spinal cord during the recovery phase. IRF8 is required for macrophage.

Fig. 1 A time-course RNA-seq analysis reveals prominent IRF8 expression in the spinal cord during the recovery phase after SCI. A time-course RNA-seq analysis.

Fig. 1 IDH3α expression is elevated in human-derived gliomas.

Fig. 4 PSMA PET/CT enables visualization of CD19-tPSMA(N9del) CAR T cell infiltration into local and metastatic tumors. PSMA PET/CT enables visualization.

Fig. 5 Schematic phase diagrams of Ising spin systems and Mott transition systems. Schematic phase diagrams of Ising spin systems and Mott transition systems.

Fig. 2 In vivo tumorigenicity of 4T1, CTC1, and DTC1 cells and EMT traits of DTC1-derived CTC lines. In vivo tumorigenicity of 4T1, CTC1, and DTC1 cells.

Fig. 4 OER performance of ACoO3 (A = Ca, Sr) in alkaline solutions with different pH. OER performance of ACoO3 (A = Ca, Sr) in alkaline solutions with.

IDH3α interacts with cSHMT during S phase at the nuclear lamina

Fig. 3 Regulation of CD73 and CD39 cell membrane expression and extracellular adenosine levels by ERs in osteoclasts. Regulation of CD73 and CD39 cell.

Fig. 2 IRF8 is expressed in CD68+ macrophages after SCI.

Fig. 1 Deficient CD73 and CD39 expressions and extracellular adenosine concentration in BM of OVX animals. Deficient CD73 and CD39 expressions and extracellular.

Fig. 2 NH3, NOx, SO2, and NMVOC emission changes triggered by the JJJ clean air policy. NH3, NOx, SO2, and NMVOC emission changes triggered by the JJJ.

Fig. 4 Effects of morphogen priming of engineered mesenchymal condensations on in vitro chondrogenic lineage specification at the time of implantation.

Fig. 4 Adenosine A2BR signaling promote osteogenic and inhibit osteoclast differentiation in vitro. Adenosine A2BR signaling promote osteogenic and inhibit.

Fig. 4 Relationships between light and economic parameters.

Fig. 5 Comparison of the liquid products generated from photocatalytic CO2 reduction reactions (CO2RR) and CO reduction reactions (CORR) on two catalysts.

Fig. 1 Location of the Jirzankal Cemetery.

Fig. 4 CO2 emission changes triggered by the JJJ clean air policy.

Fig. 6 MSC encapsulation in vitro within PdBT cross-linked gels.

Multiplexed four- and eight-channel devices for rapid processing

Fig. 2 RVFV causes pathology within the liver, uterus, and placenta of pregnant dams. RVFV causes pathology within the liver, uterus, and placenta of pregnant.

Fig. 4 Single-particle contact angle measurements.

Fig. 5 IL-5–mediated signaling is critical for the development of CD1dintCD5+ Breg precursor cells and IL-10+ Breg cells. IL-5–mediated signaling is critical.

Fig. 5 Density plots showing the relationship between growth responses to extreme events and site-level mean precipitation from all sites (N = 1314). Density.

Fig. 2 Supraballs and films from binary SPs.

Fig. 5 Reconstitution of the dormant state using fetal ovaries.

Fig. 1 Schematic structure of a fluorescently labeled eGLP1-conjugated MALAT1 ASO and internalization of fluorescent eGLP1 and eGLP1-MALAT1-ASO. Schematic.

Fig. 2 Comparison between the different reflective metasurface proposals when θi = 0° and θr = 70°. Comparison between the different reflective metasurface.

The combined signal spectra of PSD for protons and helium nuclei

Fig. 3 MCA-mediated neutrophil recruitment accelerates bacterial clearance in the skin. MCA-mediated neutrophil recruitment accelerates bacterial clearance.

Fig. 4 Light-induced TRPV1 activation promotes in vitro tubulogenesis in ECFC cultures. Light-induced TRPV1 activation promotes in vitro tubulogenesis.

Fig. 3 Minimal treatment of ETL decreases metastatic burden and prolongs survival in the 4T1 breast carcinoma model after tumor resection. Minimal treatment.

Fig. 4 Systemic AAV9 delivery of gene editing components to ΔEx44 mice rescues dystrophin expression. Systemic AAV9 delivery of gene editing components.

Fig. 5 Phototransduction mechanisms.

Fig. 5 Enhanced neuroblast migration promotes functional recovery.

Fig. 3 Calculated electronic structure of ZrCoBi.

Fig. 3 Correction of Dmd exon 44 deletion in mice by intramuscular AAV9 delivery of gene editing components. Correction of Dmd exon 44 deletion in mice.

Fig. 1 TH17 cell differentiation was severely impaired in Cxxc1-deficient mice. TH17 cell differentiation was severely impaired in Cxxc1-deficient mice.

Cxxc1-deficient TH17 cells exhibit a Treg cell–like expression profile

Presentation transcript:

Fig. 6 Activating IRF8 promotes macrophage migration during the recovery phase after SCI. Activating IRF8 promotes macrophage migration during the recovery phase after SCI. (A) Macrophage transplantation protocol. Using a cell sorter, IRF8-activated and nonactivated macrophages were isolated from injured cords at 4 and 7 dpi of EGFP+ bone marrow chimeric mice, respectively. These cells were transplanted into the spinal cord of EGFP− WT mice immediately after SCI. (B) Representative images of the immunohistochemical analysis of injured spinal cords transplanted with IRF8-activated or nonactivated macrophages. The arrowheads indicate the injection sites of macrophages. (C) Craniocaudal distribution of the transplanted IRF8-activated macrophages compared with the transplanted nonactivated macrophages in the injected spinal cord at 7 dpi (n = 8 sections/four mice per group). (D) Immunocytochemical analysis of the IRF8 translocation into the nuclei of macrophages differentiated from isolated CD11b+/CD45high/Ly6G− peripheral blood–derived monocytes 3 hours after IFN-γ and LPS combination stimulation, stained with CD68 (green), IRF8 (red), and Hoechst (blue). (E) Fluorescence ratio of nuclear IRF8 to cytosolic IRF8 (n = 30 cells per group). (F) mRNA expression of purinergic receptors in macrophages 3 hours after combination stimulation (n = 8 dishes per group). (G) Schedule of in vivo combination injection after SCI. PBS or combination was injected into the spinal cord after SCI. (H) Representative images of the immunohistochemical analysis of the injured cord receiving the combination injection. CD68+ macrophages with combination injection rapidly migrated toward the lesion epicenter at 4 dpi. Increased nuclear translocation of IRF8 of macrophages was shown in the injured cord with combination injection. Scale bars, 500 μm (left) and 10 μm (right). (I) Ratio of nuclear IRF8 to cytosolic IRF8 of macrophages in the injured cord with PBS or combination injection at 4 dpi (n = 30 cells from eight slides per eight mice per group). (J) Time course of macrophage migration in the injured cord with the combination injection. (K) Comparison of the craniocaudal distribution of WT/IRF8−/− macrophages with combination injection and those with PBS injection (n = 8 per group per time point). (L) Representative images of the immunofluorescent staining of spinal cord with PBS/combination injection at 14 dpi. (M) Quantification of the 5HT+ area of spinal cord with PBS/combination injection at 14 dpi (eight sections/eight mice per group). (N) BMS score of WT mice with PBS or combination injection (n = 8 mice per group). (O) Footprint analysis of WT/IRF8−/− mice with combination injection compared to those with PBS injection at 14 dpi. (P) Stride length of WT/IRF8−/− mice with PBS and combination injection at 14 dpi (n = 14 mice per group). (Q) Scores of the grip walk test in each mouse at 14 dpi (n = 14 per group). Scale bars, 500 μm (B and J), 10 μm (D), and 100 μm (L). The images shown in (H), (J), and (L) are representative of eight slides from eight mice/each group per time point. *P < 0.05 and **P < 0.005, Wilcoxon rank-sum test (E, F, I, and M), ANOVA with the Tukey-Kramer post hoc test (C, K, P, and Q) and two-way repeated-measures ANOVA with the Tukey-Kramer post hoc test (N). The data are presented as means ± SEM. (C, F, K, M, N, P, and Q). Kazu Kobayakawa et al. Sci Adv 2019;5:eaav5086 Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).