Impact of CMV congenital infection on the postnatal olfactory system at W3. Impact of CMV congenital infection on the postnatal olfactory system at W3.

Slides:

Advertisements

Similar presentations

Inhibition of autophagy augments 5-fluorouracil chemotherapy in human colon cancer in vitro and in vivo model Jie Li, Ni Hou, Ahmad Faried, Soichi Tsutsumi,

Advertisements

Comment on “ApoE-Directed Therapeutics Rapidly Clear β-Amyloid and Reverse Deficits in AD Mouse Models” by Karthikeyan Veeraraghavalu, Can Zhang, Sean.

Biphasic Alteration of the Inhibitory Synapse Scaffold Protein Gephyrin in Early and Late Stages of an Alzheimer Disease Model Eva Kiss, Karin Gorgas,

Transforming Growth Factor-β Signaling Curbs Thymic Negative Selection Promoting Regulatory T Cell Development Weiming Ouyang, Omar Beckett, Qian Ma,

Volume 85, Issue 2, Pages (January 2014)

Volume 7, Issue 6, Pages (December 2016)

Impact of IRE1 somatic mutations on tumor development

Human NK cell development in NOD/SCID mice receiving grafts of cord blood CD34+ cells by Christian P. Kalberer, Uwe Siegler, and Aleksandra Wodnar-Filipowicz.

Volume 11, Issue 1, Pages (July 2012)

Klotho reduction causes macrophage invasion into the CP

Origin and function of olfactory bulb interneuron diversity

Volume 13, Issue 6, Pages (November 2015)

Volume 25, Issue 4, Pages (April 2014)

Increased astrocyte proliferation is paralleled with reduced infiltration of immune cells in mice with inducible SMOM2 expression in astrocytes at 5 dpi.

Figure 2S (Supplementary Data)

Effects of GM1 on astroglial and microglial markers

Volume 19, Issue 5, Pages (November 2016)

Supplementary Figure1. Systemic administration of LPS does not lead to a general induction of EGFP+ expression in CD11b+ microglia in different regions.

Autophagy in proximal tubules protects against acute kidney injury

Volume 7, Issue 2, Pages (February 2010)

Microarray analysis of phospho-Twist1–responsive genes.

FoxO3 Regulates Neural Stem Cell Homeostasis

Volume 92, Issue 3, Pages (September 2017)

BDNF expression in the cerebellum and brain stem region.

Volume 19, Issue 10, Pages (June 2017)

Volume 48, Issue 4, Pages e4 (April 2018)

Fig. 3. Morphological changes associated with glial activation were reduced in 16-month-old APP/PS1;C3 KO mice. Morphological changes associated with glial.

Olfactory-Experience- and Developmental-Stage-Dependent Control of CPEB4 Regulates c-Fos mRNA Translation for Granule Cell Survival Ching-San Tseng,

GRM7 Regulates Embryonic Neurogenesis via CREB and YAP

Aβ Secretion and Plaque Formation Depend on Autophagy

Effects of E2 on levels of phospho-CREB in the DH of OVX females, sham males, and GDX males. Effects of E2 on levels of phospho-CREB in the DH of OVX females,

AKT2, but not AKT1, is required for regulating survival of PTEN-deficient prostate tumor spheroids. AKT2, but not AKT1, is required for regulating survival.

The Lymphocytic Choriomeningitis Virus Envelope Glycoprotein Targets Lentiviral Gene Transfer Vector to Neural Progenitors in the Murine Brain Colleen.

ILK knockdown decreases mTOR signaling in PKD kidneys.

Expression of mutant EGFR and downstream signaling components in tumors of mice at survival endpoint. Expression of mutant EGFR and downstream signaling.

Defects in neuronal cell migration from the SVZ

Cellular reaction to intraventricular DMSO injection.

OTX2 transfers into supporting cells of neurogenic niches.

Fig. 3. Enhanced EGFR signaling in Rhbdf2P159L/P159L mice.

Sustained PGC-1α loss of function triggers mesenchymal transition.

Guo-li Ming, Hongjun Song Neuron Volume 70, Issue 4, Pages (May 2011)

The complexity and the total dendritic length of ApoE4 adult-born granule cells are significantly reduced. The complexity and the total dendritic length.

Activation of glial cells.

OTX2 in RMS and V-SVZ is non-cell-autonomous.

The absence of host Fbln5 results in increased apoptosis and decreased proliferation in Pan02 tumors. The absence of host Fbln5 results in increased apoptosis.

Microglia activation in distinct sites of the spinal cord from mice with bronchial asthma or atopic dermatitis on the back skin at the thoracic spinal.

Effect of mitomycin C and bortezomib on the growth of LS174T intraperitoneal tumors. Effect of mitomycin C and bortezomib on the growth of LS174T intraperitoneal.

Effect of M-cadherin RNAi on apoptosis in confluent C2C12 myoblasts.

Immunohistochemistry for peripheral blood-born macrophage infiltration

PTZ-induced c-Fos and Arc proteins in hippocampal dentate gyrus

Chronic sleep loss is associated with microglial phagocytosis.

PTZ-induced neuronal activity visualized by IEGs

TRAF6 exacerbates I/R-induced neuronal death.

Stress-induced monocyte accumulation in the brain and the neurovascular induction of ICAM-1 were prevented by ADX. Male C57BL/6 mice were subjected to.

Impact of CMV congenital infection on olfactory discrimination in adult male mice. Impact of CMV congenital infection on olfactory discrimination in adult.

Impact of CMV congenital infection on hearing.

Topographic organization of CA3 to CA1 projections revealed through monosynaptic rabies tracing by specifically targeting CA1 pyramidal cells along the.

Impact of CMV congenital infection on olfactory perception in adult male mice. Impact of CMV congenital infection on olfactory perception in adult male.

Fig. 3. Morphological changes associated with glial activation were reduced in 16-month-old APP/PS1;C3 KO mice. Morphological changes associated with glial.

TOMA does not affect NFTs in posterior hypothalamus or cortex.

IR expression in the olfactory epithelium.

Specificity of the WDR81 antibody for the antigen.

Volume 7, Issue 6, Pages (December 2016)

Expression of the cell death markers activated caspase-3 and LC3 I/II in TUBO cells. Expression of the cell death markers activated caspase-3 and LC3 I/II.

WP1066 induces caspase-dependent apoptosis.

Fig. 5 C9orf72 knockdown disrupts autophagy induction.

Passive immunization with CT α-syn antibodies improved TH pathology and neuroinflammation in the striatum of α-syn tg mice. Passive immunization with CT.

Induction of PARP cleavage (A) and activation of caspases (B) after treatment with a combination of TRAIL and cisplatin. Induction of PARP cleavage (A)

Gdx rats exhibit increased BDNF-ir in the MF pathway.

Densin knock-out mice have normal gross neuroanatomy and low body weight. Densin knock-out mice have normal gross neuroanatomy and low body weight. A,

Presentation transcript:

Impact of CMV congenital infection on the postnatal olfactory system at W3. Impact of CMV congenital infection on the postnatal olfactory system at W3. A, Sagittal section of a murine brain showing the neurogenic dentate gyrus (DG) of the hippocampus, the neurogenic SVZ, the RMS, and the OB. Neuroblasts born in the neonatal and postnatal SVZ migrate via the RMS until the OB, where they differentiate into GCL or GL interneurons. B, D–F, Representative staining of coronal SVZ (D, bottom) and OB slices with DAPI (B, D, E) murine CMV IE1 (B, D, bottom), cleaved caspase 3 (Casp3, D, top), OMP expressed by OSN (D, middle), Iba (D, middle; E), CD68 expressed by activated microglia and macrophages (E), Ki67 (D), TH (F), CB (F), and CR (F), antibodies showing CMV+, apoptotic Casp3+, OSN, macrophages, microglia, Ki67+ neural progenitor cells, TH+, CB+ and CR+ cells in CTL and congenital CMV-infected mice at W3 after birth. C, G–I, Screening of the OB proteins from congenital CMV-infected mice at W3 for autophagy induction (C, I), cell apoptosis (C, G), and microglial reaction (C, G). Lysates were extracted from the OB of CTL and congenital CMV-infected mice at W3 and analyzed by immunoblot (C) using antibodies to detect Casp3, Iba1, CD68, Beclin1, phospho-AMPK, Atg5, p62, LC3 I/II, LAMP, phospho-mTOR, and actin (three mice each). The levels of Casp3, Iba, CD68, Beclin1, pAMPK, Atg5, p62, LC3 II/ LC3 I, LAMP, pmTOR, and actin were quantified (G,I) by band intensity with Fiji software. H, J, Ki67+, TH+, CB+, and CR+ cell densities in the SVZ, GL, glomerulus (glom), and GCL at W3 following congenital CMV inoculation. All mice are W3-old males injected at E13 with PBS (CTL) or CMV. For immunoblot analysis (G, I), n = 4 mice per group. For cell density analysis (H) and area ratio (J), n = 4–6 mice per group. For glom size (J), n = 409 glom from 4 CTL, n = 352 glom from 4 CMV. Results are shown as mean ± SEM. p-values were calculated by Mann–Whitney test. *p < 0.05, **p < 0.01. Scale bars: 100 μm in D, bottom; 50 μm in B, D, middle, and F; 25 μm in D, bottom; and 5 μm in D, top, and E. Françoise Lazarini et al. J. Neurosci. 2018;38:10424-10437 ©2018 by Society for Neuroscience