Amoeba culturing methods for recovering amoeba-resisting microorganisms (ARMs). Amoeba culturing methods for recovering amoeba-resisting microorganisms.

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Amoeba culturing methods for recovering amoeba-resisting microorganisms (ARMs). Amoeba culturing methods for recovering amoeba-resisting microorganisms (ARMs). On the left, the method is performed by using laboratory axenic amoebal strains for recovering ARMs from diverse samples. On the right, the amoebae are wild species isolated from the samples, and the ARMs are recovered after amoebal isolation. The circles represent agar plates coated with bacteria, the line is the inoculation point of the sample, and the squiggles represent the growth of the amoebae, following a migration front. BCYE, buffered charcoal-yeast extract. Jean-Christophe Lagier et al. Clin. Microbiol. Rev. 2015; doi:10.1128/CMR.00110-14