Spin diffusion buildup from bound water to amide protons in NaK2K

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Fig. 3 Spin diffusion buildup from bound water to amide protons in NaK2K. Spin diffusion buildup from bound water to amide protons in NaK2K. 2D HN slices with mixing times of 5 ms (A) and 35 ms (B) taken at the chemical shift of bound water (4.86 ppm) from 3D H(H)HN spin diffusion experiments. (C) Close up around the SF in a monomer of the tetrameric NaK2K structure (PDB ID: 3ouf). The magnetization transfer from bound water molecules to amide protons in the SF is indicated as orange spheres, where larger spheres represent faster spin diffusion buildup rates. S1 to S4 represent the binding sites in the SF. (D) Spin diffusion buildup plots from bound water to amide protons for the indicated residues. The error bars are based on the average noise levels in the 3D spectra. A.U., arbitrary unit; SD, spin diffusion. Carl Öster et al. Sci Adv 2019;5:eaaw6756 Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).