Expression of p130CAS in primary culture neurons

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Expression of p130CAS in primary culture neurons Expression of p130CAS in primary culture neurons. a–f, Expression of DCC (a, d) and p130CAS (b, e) in primary cortical neurons from E15 mice. c is the merged image of a and b. f is the merged image of d and e. d, e, and f are higher-magnification images of ... Expression of p130CAS in primary culture neurons. a–f, Expression of DCC (a, d) and p130CAS (b, e) in primary cortical neurons from E15 mice. c is the merged image of a and b. f is the merged image of d and e. d, e, and f are higher-magnification images of the boxed regions in a, b, and c, respectively. Neurons from E15 mice cortex were cultured overnight and immunostained with anti-DCC (G97–449) and anti-p130CAS (SC-860). Scale bar, 10 μm. g–m, Expression of DCC (g, j) and p130CAS (h, k) in E13 primary spinal cord neurons. i is the superimposed images of g and h. l is the superimposed image of j and k. m is the phase image of j, k, and l. j–m are higher-magnification images showing DCC and p130CAS expression in the growth cone of embryonic spinal cord neurons. Scale bar, 10 μm. n–o, Expression of p130CAS in E13 mice spinal cord. o is the higher-magnification image of the boxed region of image n. p130CAS is strongly expressed in the commissural axons as they project toward the floor plate (white arrow), the ventral commissural region (VC; arrowhead), and lateral fasciculus (LF; green arrow). Scale bar, 100 μm. Guofa Liu et al. J. Neurosci. 2007;27:957-968 ©2007 by Society for Neuroscience