Last class Cloning Transformation Paper 2 intro.

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Last class Cloning Transformation Paper 2 intro

Plasmid prep Plasmid in cells How to get out?

Plasmid prep

Plasmid prep: 2 Precipitate Proteins & DNA

Plasmid prep: 3 Bind plasmid

Plasmid prep: 3 Bind plasmid

Plasmid prep: 3 Bind plasmid

Plasmid prep: 4 Elute plasmid

Plasmid prep & RE mapping Lyse bacteria, isolate plasmid pBK-CMV with gene RE digest and run on a gel Determine RE map of plasmid (size of insert = 5.5Kb)

Implied knowledge! What were some things you had to look up?

First experiments Purpose? Experiment? Results?

First experiments Purpose? Experiment? Results? Next step?

c-Myc? Purpose? Experiment? Results?

Control: Prediction for iPSC expression pattern Purpose? Experiment? Prediction for miR iPSCs?

Control: Prediction for exogenous genes? Purpose? Experiment? Prediction for miR iPSCs? What else?

miRNA iPSCs? Purpose? Experiment? Prediction for miR iPSCs?

ChIP Y Y Unbind & sequence

ChIPs? Purpose? Experiment? Result?

ChiPs + Expression = ?

% GFP +ve colonies = ? Purpose? Experiment? Results?