Kyung Hee Chang, MD, PhD, Daniel T

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Presentation transcript:

Novel 16-minute technique for evaluating melanoma resection margins during Mohs surgery Kyung Hee Chang, MD, PhD, Daniel T. Finn, MD, Dennis Lee, MD, Jag Bhawan, MD, Gerard E. Dallal, PhD, Gary S. Rogers, MD Journal of the American Academy of Dermatology Volume 64, Issue 1, Pages 107-112 (January 2011) DOI: 10.1016/j.jaad.2010.02.055 Copyright © 2010 American Academy of Dermatology, Inc. Terms and Conditions

Fig 1 Diagram of MMS for lentigo maligna processed with 16-minute automated MART-1 stains. Negative control tissue is taken from sun-exposed skin, and positive control tissue from the clinically obvious tumor-bearing skin in the Mohs layer. Journal of the American Academy of Dermatology 2011 64, 107-112DOI: (10.1016/j.jaad.2010.02.055) Copyright © 2010 American Academy of Dermatology, Inc. Terms and Conditions

Fig 2 Frozen section H&E stain (A), permanent section H&E stain (B), permanent section MART-1 stain (C), and frozen section 16-minute automated MART-1 stain (D) of negative control skin. Journal of the American Academy of Dermatology 2011 64, 107-112DOI: (10.1016/j.jaad.2010.02.055) Copyright © 2010 American Academy of Dermatology, Inc. Terms and Conditions

Fig 3 Comparison of the readability of permanent H&E and 16-minute MART-1 stains within the Mohs surgeon group. Mohs surgeons found the rapid MART-1 stain significantly easier to read compared with conventional permanent sections stained with H&E. Journal of the American Academy of Dermatology 2011 64, 107-112DOI: (10.1016/j.jaad.2010.02.055) Copyright © 2010 American Academy of Dermatology, Inc. Terms and Conditions