Quantitative analysis of effects of the Egr inhibitor construct on neurite outgrowth. Quantitative analysis of effects of the Egr inhibitor construct on.

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Quantitative analysis of effects of the Egr inhibitor construct on neurite outgrowth. Quantitative analysis of effects of the Egr inhibitor construct on neurite outgrowth. A, PC12 cells expressing GFP were scored as either neurite bearing or not. Exposure to NGF elicits neurite outgrowth in ∼80% of cells, indicating that expression of the GFP construct does not alter this response to NGF. Cotransfection with the Egr inhibitor construct markedly reduced the percentage of neurite-bearing cells observed after NGF treatment (NGF +Δ1-249). B, The Egr inhibitor construct does not reduce the percentage of neurite-bearing cells induced by exposure to db-cAMP (dbcAMP + Δ1-249).C, GFP-positive cells were grouped according to the number of neurites present after NGF treatment. Cotransfection with the Egr inhibitor construct produces a marked shift toward lower neurite number. D, The Egr inhibitor construct does not affect the distribution of neurite number after db-cAMP treatment.Values shown in C differ slightly from those in A because they are based on independent counts. Data presented in this figure are from cells counted 2 d after NGF or db-cAMP treatment. Over 500 cells were scored for each experimental condition. Yechiel Levkovitz et al. J. Neurosci. 2001;21:45-52 ©2001 by Society for Neuroscience