Interobserver and intraobserver variation in day 3 embryo grading Allison E. Baxter Bendus, M.S., Jacob F. Mayer, Ph.D., Sharon K. Shipley, M.S., William H. Catherino, M.D., Ph.D.  Fertility and Sterility  Volume 86, Issue 6, Pages 1608-1615 (December 2006) DOI: 10.1016/j.fertnstert.2006.05.037 Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

FIGURE 1 Mean variation of interobserver grading by embryologist. The 25 embryologists were compared to the grades provided by the creator of the five category grading system (7), who served as the control (L.V.). There were 35 embryos evaluated by each embryologist. This figure demonstrates the mean variation and the SE of the mean in grading for each embryologist compared to control. Baxter Bendus. Variation of day 3 embryo grading. Fertil Steril 2006. Fertility and Sterility 2006 86, 1608-1615DOI: (10.1016/j.fertnstert.2006.05.037) Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

FIGURE 2 Mean variation of interobserver grading by number of cycles per year. There were 13 embryologists who were in programs that performed less than 500 cycles per year, and 13 who were in programs that performed more than 500 cycles per year (Table 1). The greater mean variation in interobserver grading correlated with fewer cycles (P<.001). Baxter Bendus. Variation of day 3 embryo grading. Fertil Steril 2006. Fertility and Sterility 2006 86, 1608-1615DOI: (10.1016/j.fertnstert.2006.05.037) Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

FIGURE 3 The mean variation of intraobserver grading by embryologist. The 26 embryologists graded seven duplicate video clips of embryos and graded each according to the grading system described (7). This figure shows the mean variation and the SE of the mean in grading for all duplicates. Baxter Bendus. Variation of day 3 embryo grading. Fertil Steril 2006. Fertility and Sterility 2006 86, 1608-1615DOI: (10.1016/j.fertnstert.2006.05.037) Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions