Gastrointestinal digestion of Bet v 1–homologous food allergens destroys their mediator- releasing, but not T cell–activating, capacity Eva Maria Schimek,

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Presentation transcript:

Gastrointestinal digestion of Bet v 1–homologous food allergens destroys their mediator- releasing, but not T cell–activating, capacity Eva Maria Schimek, MD, Bettina Zwölfer, Peter Briza, PhD, Beatrice Jahn-Schmid, PhD, Lothar Vogel, PhD, Stefan Vieths, PhD, Christof Ebner, MD, Barbara Bohle, PhD Journal of Allergy and Clinical Immunology Volume 116, Issue 6, Pages 1327-1333 (December 2005) DOI: 10.1016/j.jaci.2005.09.007 Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions

Fig 1 Pepsin and trypsin digestion profiles of PR-10–like allergens. M, Marker; lane 0, untreated allergen; lane E, both enzymes alone; lanes 1-3, allergen plus pepsin for 1, 5, and 30 seconds; lanes 4-7, allergen plus trypsin for 1, 5, 15, and 30 minutes; lanes 8-11, allergen plus trypsin for 30, 45, 60, and 120 minutes. The upper panels show the Coomassie staining of a 20% gel. The lower panels depict the IgE binding of a serum pool of 10 individuals with birch pollen allergy, as determined by means of immunoblotting. Journal of Allergy and Clinical Immunology 2005 116, 1327-1333DOI: (10.1016/j.jaci.2005.09.007) Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions

Fig 2 Mediator release induced by digested allergens. RBL cells were loaded with serum IgE from 3 different individuals with OAS to apples, hazelnuts, and celery. The percentage of mediator release (x-axis) induced by equal concentrations of intact allergens (filled circles) and allergens after gastrointestinal digestion (open circles) is shown. Journal of Allergy and Clinical Immunology 2005 116, 1327-1333DOI: (10.1016/j.jaci.2005.09.007) Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions

Fig 3 Proliferative responses to digested allergens. PBMCs of allergic (upper panel) and nonallergic (lower panel) individuals were stimulated with entire allergens and allergens after gastrointestinal digestion. Dpm, Delta cpm. Background levels ranged from 893 to 6566 cpm in allergic individuals and from 1215 to 8086 cpm in healthy individuals. ∗P < .05, Mann-Whitney U test. Journal of Allergy and Clinical Immunology 2005 116, 1327-1333DOI: (10.1016/j.jaci.2005.09.007) Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions

Fig 4 Proteolytic fragments detected after gastrointestinal digestion of allergens. Peptides of greater than 7 amino acid residues are highlighted in gray in the sequence of each allergen. Amino acids identical with Bet v 1 are indicated in bold, and homologous residues are indicated in italics. The frames highlight known T-cell epitopes of Bet v 1 matching proteolytic fragments. Journal of Allergy and Clinical Immunology 2005 116, 1327-1333DOI: (10.1016/j.jaci.2005.09.007) Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions