Comparison of ciliary activity and inflammatory mediator release from bronchial epithelial cells of nonatopic nonasthmatic subjects and atopic asthmatic.

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Presentation transcript:

Comparison of ciliary activity and inflammatory mediator release from bronchial epithelial cells of nonatopic nonasthmatic subjects and atopic asthmatic patients and the effect of diesel exhaust particles in vitro Hasan Bayram, MDa, Jagdish L. Devalia, PhDa, Omar A. Khair, MBa, Muntasir M. Abdelaziz, MBa, Raymond J. Sapsford, MISTa, Masaru Sagai, PhDb, Robert J. Davies, MDa Journal of Allergy and Clinical Immunology Volume 102, Issue 5, Pages 771-782 (November 1998) DOI: 10.1016/S0091-6749(98)70017-X Copyright © 1998 Mosby, Inc. Terms and Conditions

Fig. 1 Typical confluent human bronchial epithelial cell culture after 2 to 3 weeks in culture. Journal of Allergy and Clinical Immunology 1998 102, 771-782DOI: (10.1016/S0091-6749(98)70017-X) Copyright © 1998 Mosby, Inc. Terms and Conditions

Fig. 2 Typical human bronchial epithelial cell culture: A , negative control; B , stained for cytokeratin. Journal of Allergy and Clinical Immunology 1998 102, 771-782DOI: (10.1016/S0091-6749(98)70017-X) Copyright © 1998 Mosby, Inc. Terms and Conditions

Fig. 3 Effect of DEP on the CBF of A, nonasthmatic (n = 7) and B, asthmatic (n = 10) bronchial epithelial cell cultures after incubation for 2 to 24 hours. Results are calculated as percent change from baseline in each culture and expressed as median and interquartile ranges (*P < .05 and **P < .01 vs 0 μg/mL DEP at all time points). Journal of Allergy and Clinical Immunology 1998 102, 771-782DOI: (10.1016/S0091-6749(98)70017-X) Copyright © 1998 Mosby, Inc. Terms and Conditions

Fig. 4 Constitutive release of IL-8, GM-CSF, RANTES, and sICAM-1 from bronchial epithelial cell cultures of nonasthmatic subjects (shaded circles) and asthmatic patients(filled circles) after incubation for 24 hours. Results are expressed as median and individual values. ND Not detected. Journal of Allergy and Clinical Immunology 1998 102, 771-782DOI: (10.1016/S0091-6749(98)70017-X) Copyright © 1998 Mosby, Inc. Terms and Conditions

Fig. 5 Effect of DEP on release of IL-8 from A, nonasthmatic, and B, asthmatic bronchial epithelial cell cultures after incubation for 24 hours. Results are expressed as median and individual values (all asthmatic bronchial epithelial cell cultures served as controls before incubation with DEP, as described in text). Journal of Allergy and Clinical Immunology 1998 102, 771-782DOI: (10.1016/S0091-6749(98)70017-X) Copyright © 1998 Mosby, Inc. Terms and Conditions

Fig. 6 Effect of DEP on release of GM-CSF from A , nonasthmatic, and B , asthmatic bronchial epithelial cells after incubation for 24 hours. Results are expressed as median and individual values (all asthmatic bronchial epithelial cell cultures served as controls before incubation with DEP, as described in text). Journal of Allergy and Clinical Immunology 1998 102, 771-782DOI: (10.1016/S0091-6749(98)70017-X) Copyright © 1998 Mosby, Inc. Terms and Conditions

Fig. 7 Effect of DEP on release of RANTES from asthmatic bronchial epithelial cell cultures after incubation for 24 hours. Results are expressed as median and individual values (bronchial epithelial cell cultures served as controls before incubation with DEP, as described in text). Journal of Allergy and Clinical Immunology 1998 102, 771-782DOI: (10.1016/S0091-6749(98)70017-X) Copyright © 1998 Mosby, Inc. Terms and Conditions

Fig. 8 Effect of DEP on release of sICAM-1 from A , nonasthmatic, and B , asthmatic bronchial epithelial cells cultures after incubation for 24 hours. Results are expressed as median and individual values (all asthmatic bronchial epithelial cell cultures served as controls before incubation with DEP, as described in text). Journal of Allergy and Clinical Immunology 1998 102, 771-782DOI: (10.1016/S0091-6749(98)70017-X) Copyright © 1998 Mosby, Inc. Terms and Conditions