Fig. 1. siRNA-mediated suppression of Shank3, Nlgn3, Fmr1, Mecp2 or Tsc1 in the dorsal striatum produced mild to severe autistic-like phenotypes. (A) Experimental.

Slides:

Advertisements

Similar presentations

Fig. 2. The expression of pKr-2 in the substantia nigra (SN) of patients with Parkinson's disease (PD). (A, B) Western blot analysis for pKr-1-2 and pKr-2,

Advertisements

From: Propofol Limits Microglial Activation after Experimental Brain Trauma through Inhibition of Nicotinamide Adenine Dinucleotide Phosphate Oxidase Anesthes.

Morozova A.Y., Zubkov E.A., Chekhonin V.P.

Neal et al.Supplementary Figure S1

Nm23-H1 Regulates Glucose-Stimulated Insulin Secretion in Pancreatic β-Cells via Arf6-Rac1 Signaling Axis Cell Physiol Biochem 2013;32: DOI: /

Figure 1 Body weight of control and BPA-treated mothers after delivery

Volume 23, Issue 13, Pages (June 2018)

Linking Cholinergic Interneurons, Synaptic Plasticity, and Behavior during the Extinction of a Cocaine-Context Association Junuk Lee, Joel Finkelstein,

Canonical Wnt/β-catenin signaling mediates transforming growth factor-β1-driven podocyte injury and proteinuria Dan Wang, Chunsun Dai, Yingjian Li, Youhua.

Volume 66, Issue 6, Pages (June 2010)

Volume 9, Issue 5, Pages (December 2014)

The Troubled Touch of Autism

Essential Role of Presynaptic NMDA Receptors in Activity-Dependent BDNF Secretion and Corticostriatal LTP Hyungju Park, Andrei Popescu, Mu-ming Poo

Fig. 1. BCAS1 expression identifies newly generated oligodendrocytes.

Multiple Actions of Spinophilin Regulate Mu Opioid Receptor Function

Laminin γ2 Mediates Wnt5a-Induced Invasion of Gastric Cancer Cells

Supplemental Figure S1. Expression of MYOCD, MYOCD ceRNA and miRNAs

Volume 8, Issue 2, Pages (July 2014)

Linking Cholinergic Interneurons, Synaptic Plasticity, and Behavior during the Extinction of a Cocaine-Context Association Junuk Lee, Joel Finkelstein,

Volume 23, Issue 8, Pages (May 2018)

Dexmedetomidine-mediated neuroprotection against sevoflurane-induced neurotoxicity extends to several brain regions in neonatal rats J.F. Perez-Zoghbi,

Volume 83, Issue 5, Pages (September 2014)

Adult (2- to 4-mo-old) Nlgn3y/– mice modify the social behavior of their littermates. Adult (2- to 4-mo-old) Nlgn3y/– mice modify the social behavior of.

Volume 22, Issue 1, Pages (January 1999)

The maternal interleukin-17a pathway in mice promotes autismlike phenotypes in offspring by Gloria B. Choi, Yeong S. Yim, Helen Wong, Sangdoo Kim, Hyunju.

Volume 23, Issue 3, Pages (February 2013)

Behavioral Disturbances in Estrogen-Related Receptor alpha-Null Mice

Volume 54, Issue 2, Pages (April 2007)

Volume 72, Issue 8, Pages (October 2007)

μ-Opioid Receptor and CREB Activation Are Required for Nicotine Reward

Volume 12, Issue 4, Pages (October 2005)

Volume 19, Issue 2, Pages (April 2017)

Essential Role of Presynaptic NMDA Receptors in Activity-Dependent BDNF Secretion and Corticostriatal LTP Hyungju Park, Andrei Popescu, Mu-ming Poo

Volume 18, Issue 5, Pages (January 2017)

Corticostriatal Transmission Is Selectively Enhanced in Striatonigral Neurons with Postnatal Loss of Tsc1 Katelyn N. Benthall, Stacie L. Ong, Helen S.

Rational Design of Therapeutic siRNAs: Minimizing Off-targeting Potential to Improve the Safety of RNAi Therapy for Huntington's Disease Ryan L Boudreau,

Supplementary Figure 5: Effect of S48168 on normalized organ and skeletal muscle weights after 12 weeks of treatment for all the experimental groups from.

by Jae-Ick Kim, Subhashree Ganesan, Sarah X

Sorting Nexin 27 Regulation of G Protein-Gated Inwardly Rectifying K+ Channels Attenuates In Vivo Cocaine Response Michaelanne B. Munoz, Paul A. Slesinger

Volume 21, Issue 9, Pages (November 2017)

Fig. 3 Exercise training enhances neuronal activity in vivo.

Volume 5, Issue 2, Pages (August 2015)

CREB1 promotes the induction of endogenous ERα target genes.

Hepatocyte Growth Factor Regulates the miR-206-HDAC4 Cascade to Control Neurogenic Muscle Atrophy following Surgical Denervation in Mice Wooshik Choi,

Fig. 4. Reduction of apoptosis and autophagic stress by treatment with silibinin in the KA-treated hippocampus. (A, B) Double-immunofluorescence staining.

Fig. 3. Neuroprotective effects of silibinin against KA-induced excitotoxicity. (A) Silibinin was intraperitoneally injected for 8 days, starting 1 day.

Fig. 5. Anti-inflammatory effects of silibinin on KA treatment in the hippocampus. (A) Representative coronal sections of the hippocampal CA1 region following.

PTZ-induced neuronal activity visualized by IEGs

MRNA Vaccine with Antigen-Specific Checkpoint Blockade Induces an Enhanced Immune Response against Established Melanoma Yuhua Wang, Lu Zhang, Zhenghong.

Fig. 2. Autistic-like phenotypes induced by the striatal inhibition of MeCP2 or TSC1 were rescued by rebalancing glutamate or dopaminergic activity. (A)

Fig. 3. Inhibition of HIF1α with chrysin induced cell death and suppressed the expressions of glycolysis-regulating genes in GBMs. (A~D) Cell viability.

Volume 17, Issue 3, Pages (October 2016)

Fig. 1. Schematic diagram of behavioral procedures

Fig. 3. Effects of RP on SPS-CF stress-induced memory impairment of spatial memory. (A) Latency to find hidden platform in Morris water maze during 5 daily.

Fig. 2. TH expression is decreased in mice with HFD-induced obesity

Fig. 2. Disease state-specific alteration of activity-dependent BDNF secretion from cortical axons. (A) Average fluorescence changes with time (ΔFt/F0)

Fig. 4. Chronic microwave treatment produced changes in glucose and corticosterone levels in blood. (A) Blood glucose levels of mice subjected to chronic.

Fig. 10. Summary of astrocyte specificity and coverage of hGFAP-CreERT2 in various brain regions. (A) Schematic sagittal section diagram shows heterogeneous.

Fig. 3. KMS99220 inhibits activation of NFκB signaling via HO-1 induction. (A) BV2 cells were treated with 10 µM KMS99220 for 1 h, and then treated with.

Fig. 3. LPS induces degeneration of DA neurons and microglial activation in the SN in vivo. LPS (5 µg/3 µl) was unilaterally injected into the SN in the.

Yonghong Chen, Shujuan Zheng, Luis Tecedor, Beverly L. Davidson

Fig. 4. Dopaminergic signaling pathways are impaired in Ewsr1 KO (−/−) mice at 3 weeks of age. (A) qPCR analysis showed that Th mRNA was significantly.

Fig. 3. hALDH1L1 promoter-driven Cre recombinase-mediated tdTomato expression in BLA. (A) Low magnification images of injection area in amygdala. Each.

Fig. 2. E-LTP is impaired in Rev-erbα KO mice during the subjective night. (A) The input-output relationships at SC-CA1 synapses showed no significant.

Fig. 1. Differential activity-induced secretion of overexpressed BDNF from cortical axons or striatal neurites in the normal or Q140 heterozygote mice.

Fig. 1. The brain of Octopus minor. (A) Left: a live specimen of O

Fig. 5. Phagocytosis of zymosan particles by BV2 cells

Fig. 1. Genetic confimation of hGFAP-CreERT2 mouse line and its tamoxifen inducible Cre expression. (A) Genetic map of hGFAP-CreERT2. (B) The primer sequences.

The effect of TGFβ on the post-RT increase of Tregs in tumors.

Volume 66, Issue 6, Pages (June 2010)

Presentation transcript:

Fig. 1. siRNA-mediated suppression of Shank3, Nlgn3, Fmr1, Mecp2 or Tsc1 in the dorsal striatum produced mild to severe autistic-like phenotypes. (A) Experimental design of siRNA injection into the dorsal striatum and following behavioral tests. A diagram showing siRNA injection sites in the dorsal striatum (AP, +1.0; ML, ±1.5; DV, −3.6 mm). Representative photomicrograph showing the dorsal striatum injected with fluorescence-tagged siRNA-control. Brain sections were prepared 2 days after siRNA injection. dST: dorsal striatum; cc: corpus callosum. (B) Real-time PCR data showing the level of siRNA-induced knockdown of Shank3, Nlgn3, Fmr1, Mecp2 and Tsc1 in HT22 cells. Cells were harvested 24 h after siRNA transfection. (C, D) Grooming (C) and digging (D) behaviors of mice with the knockdown of Shank3, Nlgn3, Fmr1, Mecp2 or Tsc1 in the dorsal striatum. (E, F) Experimental design of the social novelty preference test using U-field two-choice field (E). The exploration time of mice with the knockdown of Shank3, Nlgn3, Fmr1, Mecp2 or Tsc1in the dorsal striatum (F). (G, H) Experimental design of novel object preference (G), and sniffing time against the object of mice with the knockdown of Shank3, Nlgn3, Fmr1, Mecp2 or Tsc1 in the dorsal striatum (H). n=6–8 animals. (I) Real-time PCR data showing the siRNA-induced knockdown of Mecp2 and Tsc1 in the dorsal striatum of WT mice (I). n=3 animals, and 3 PCR repeats for each siRNA. (J, K) Experimental design of the three chamber test (J). Social interaction time between stranger vs. empty cage for mice with the knockdown of Mecp2 and Tsc1 in the dorsal striatum (K). (L, M) Locomotion in the open field test (L), and marble burying (M) behaviors of mice with the knockdown of Mecp2 and Tsc1 in the dorsal striatum. ** in Figure 1M denotes the difference between Con-siRNA and Tsc1-siRNA at p<0.01 at time points marked by arrowheads. n=6–9 animals. Data are presented as mean±SEM. * and ** denote the differences between the indicated groups at p<0.05 and p<0.01, respectively (Student's t-test, two-way ANOVA, two-way repeated measures ANOVA and Holm-Sidak post hoc test). Fig. 1. siRNA-mediated suppression of Shank3, Nlgn3, Fmr1, Mecp2 or Tsc1 in the dorsal striatum produced mild to severe autistic- like phenotypes. (A) Experimental design of siRNA injection into the dorsal striatum and following behavioral tests. A diagram showing siRNA injection… Exp Neurobiol. 2018 Dec;27(6):539-549. https://doi.org/10.5607/en.2018.27.6.539