Fig. 2 Expressions of cll, abd-A, and Abd-B by qRT-PCR.

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Fig. 5 Correlation of RNA expression and protein abundance.

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Fig. 2 Expressions of cll, abd-A, and Abd-B by qRT-PCR. Expressions of cll, abd-A, and Abd-B by qRT-PCR. (A) Relative expressions of cll, abd-A, and Abd-B in larval epidermis using the same sampling regions and time points as in the RNA-seq experiment. n = 3. Error bars indicate SD. Tukey’s test, *P < 0.05 and **P < 0.01. (B) Relative expressions of abd-A and Abd-B in larval segments T3 to A6. The samples were dissected as shown in fig. S5. The expression of a certain gene in a particular segment was calculated as the sum of the relative expressions of that gene within that particular segment. n = 3. Error bars indicate SD. (C) The sampling regions used for detecting the precise gene expression in different color patterns. (D) Relative expressions of abd-A and Abd-B by presumptive fifth color patterns during the fourth instar. The expression of a certain gene in a particular color pattern was calculated as the sum of the relative expressions of that gene within that color pattern. Error bars indicate SD. Student’s t test, *P = 0.0325. rpL3 gene was used as the inner control. D, the dorsal region that is black and lies between segments A2 and A3; W, the white region; M, the region that lies across the white region and the presumptive V-shaped marking across segments A4 and A5; G, the region that lies next to the posterior side of the presumptive V-shaped marking in segment A5; A, anterior; P, posterior. H. Jin et al. Sci Adv 2019;5:eaav7569 Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).