Fig. 1 TRPV1 immunoexpression in cell lines.

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Fig. 1 TRPV1 immunoexpression in cell lines. TRPV1 immunoexpression in cell lines. (A) Immunoblot of lysates from kidney cell lines using TRPV1 antisera (cat# ACC-030; Alomone Labs). At ~95 kDa, the expected molecular mass of TRPV1, KPT2, and KPT11 cells exhibited weak band densities, while KDT3 and MDCT cells had strong band densities. At ~42 kDa, immunolabeling for actin served as a loading control. (B) TRPV1 immunofluorescence was detected in KPT11 and MDCT cells, with negligible fluorescence in KPT2 and KDT3 cells. (C) Robust TRPV1 immunofluorescence is present in KPT2-Trpv1, but not in control KPT2-pBabe, cells. Scale bars, 20 μm. Meiyan Jiang et al. Sci Adv 2019;5:eaaw1836 Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).