Fig. 5 Analysis of VLPs formed from deletion CP mutants.

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Fig. 5 Analysis of VLPs formed from deletion CP mutants. Analysis of VLPs formed from deletion CP mutants. (A) Linear schematic representation of purified deletion mutants, based on the structure of VLP CP (N-terminal region, red; core, gray; C-terminal region, blue). SDS-PAGE (SDS–polyacrylamide gel electrophoresis) gels were obtained from soluble fractions (S) and pellets (P) after bacterial lysis and final purified soluble samples (F) of (B) N-terminal deletion mutants, (C) C-terminal deletion mutants, and (D) double deletion mutants. Negative-stain TEM micrographs show the presence or absence of filaments in various samples. The bottom part of (B) shows cryo-EM micrograph of ΔN49 (left), close-up side view of four stacked rings of ΔN49 (middle), and two-dimensional (2D) class average of the top view of ΔN49 (right). Andreja Kežar et al. Sci Adv 2019;5:eaaw3808 Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).