Mast cell and microglial proliferation in the developing POA

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Mast cell and microglial proliferation in the developing POA Mast cell and microglial proliferation in the developing POA. A, Labeling of proliferating cells (green, BrdU labeled), microglia (red, Iba1 labeled), mast cells (purple, 5-HT labeled), and merged images (right) in the POA of the newborn animal on P0 (dotte... Mast cell and microglial proliferation in the developing POA. A, Labeling of proliferating cells (green, BrdU labeled), microglia (red, Iba1 labeled), mast cells (purple, 5-HT labeled), and merged images (right) in the POA of the newborn animal on P0 (dotted white lines indicate third ventricle and leptomeninges. There was no evidence of mast cell proliferation in the neonatal POA. B, Males had more microglia than females in the POA on P2. Although there were no mast cells that colabeled with BrdU in any group (Fig. 1H), Iba1-positive microglia colocalize with BrdU in the POA on P2, indicating microglial proliferation, and males have higher numbers of proliferating microglia than females. C, Representative image of IHC to double label microglia (Iba1 stained with DAB; brown) and proliferating cells (BrdU stained with Ni-DAB; black) in the POA on P3 (3V = third ventricle). D, Representative images and Z-stack of immunofluorescently stained BrdU-positive (green) and Iba1-positive (red) cells showing colocalization. Counts of proliferating microglia were performed on DAB-stained tissue as seen in C and colocalization confirmed via confocal imaging (D). *p < 0.05. Group sizes: A: n = 4/group. B, ♂, n = 11; ♀, n = 8. Kathryn M. Lenz et al. J. Neurosci. 2018;38:8044-8059 ©2018 by Society for Neuroscience