The trimeric coiled‐coil HSBP1 protein promotes WASH complex assembly at centrosomes MDA‐MB‐231 cells expressing HaloTag WASH or CCDC53 were stained with.

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The trimeric coiled‐coil HSBP1 protein promotes WASH complex assembly at centrosomes MDA‐MB‐231 cells expressing HaloTag WASH or CCDC53 were stained with the fluorescent TMR ligand, HSBP1, and γ‐tubulin antibodies and DAPI to stain nuclei. WASH and CCDC53 colocalize with HSBP1 at the centrosome. HSBP1 is also associated with centrosomes in tissue sections from human breast. The HSBP1 staining is more elongated in shape in normal tissue than in mammary carcinomas. Seven biopsies of breast tumors displaying both carcinoma and adjacent normal tissue were tested, and they all gave similar staining. Scale bars: 10 μm. IF THIS IMAGE HAS BEEN PROVIDED BY OR IS OWNED BY A THIRD PARTY, AS INDICATED IN THE CAPTION LINE, THEN FURTHER PERMISSION MAY BE NEEDED BEFORE ANY FURTHER USE. PLEASE CONTACT WILEY'S PERMISSIONS DEPARTMENT ON PERMISSIONS@WILEY.COM OR USE THE RIGHTSLINK SERVICE BY CLICKING ON THE 'REQUEST PERMISSIONS' LINK ACCOMPANYING THIS ARTICLE. WILEY OR AUTHOR OWNED IMAGES MAY BE USED FOR NON-COMMERCIAL PURPOSES, SUBJECT TO PROPER CITATION OF THE ARTICLE, AUTHOR, AND PUBLISHER. EMBO J, Volume: 37, Issue: 13, First published: 29 May 2018, DOI: (10.15252/embj.201797706)