co-IP of LEDGF/p75 and MeCP2.

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co-IP of LEDGF/p75 and MeCP2. co-IP of LEDGF/p75 and MeCP2. A, 293T cells cotransfected with Flag-MeCP2 and eGFP-LEDGF/p75, eGFP-HIV-IN, or eGFP-BRD4-Ct expression constructs were lysed 24 hours posttransfection. Proteins in cell lysates were immunoprecipitated (IP) with antibody against GFP, resolved by SDS-PAGE, and detected by immunoblotting using anti-GFP and anti-Flag antibodies. B, endogenous proteins in U2OS cell lysates were immunoprecipitated with mouse monoclonal anti-LEDGF/p75 antibody, as well as an irrelevant IgG as negative control. Immunoprecipitated proteins were detected by immunoblotting with rabbit anti-LEDGF/p75 and anti-MeCP2 antibodies. *, degraded MeCP2. Protein input was determined by immunoblotting of whole-cell extracts. C, proteins in lysates from PC3 cells or cells stably overexpressing LEDGF/p75 (PC3p75) were immunoprecipitated with rabbit anti-MeCP2 and mouse monoclonal anti-LEDGF/p75 antibodies. Immunoprecipitated proteins were detected by immunoblotting with rabbit anti-LEDGF/p75 and anti-MeCP2 antibodies. D, endogenous proteins in PC3 cell lysates were immunoprecipitated with rabbit anti-MeCP2 antibody, as well as an irrelevant IgG as negative control. Immunoprecipitated proteins were detected by immunoblotting with anti-LEDGF/p75 and anti-MeCP2 antibodies. E, immunoblot of total proteins from PC3 cells with (sip75) and without (siSD) siRNA-mediated knockdown, using rabbit antibodies to LEDGF/p75 and MeCP2. WB, Western blotting. Lai Sum Leoh et al. Mol Cancer Res 2012;10:378-391 ©2012 by American Association for Cancer Research