Fig. 6 Local COX-2 overexpression targeted CD90+ mSSCs to accelerate bone repair. Local COX-2 overexpression targeted CD90+ mSSCs to accelerate bone repair.

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Fig. 6 Local COX-2 overexpression targeted CD90+ mSSCs to accelerate bone repair. Local COX-2 overexpression targeted CD90+ mSSCs to accelerate bone repair. (A) B6 mice were subjected to tibial fracture surgery. Immediately after the fracture surgery and on the following two consecutive days, the animals subcutaneously received a daily dose of either PBS or the depleting anti-CD90 mAb (clone 30H12) near fracture sites (±mAb). The Ab doses were 200, 100, and 100 μg on days 0, 1, and 2, respectively. Ten days after the fracture surgery, the fractured bones were harvested and the MNCs were isolated for analysis of the expressions of CD90 and CD11b by FACS. Data are shown in (B). (B) Representative FACS plots show the frequencies of CD90+ and CD11b+ cells. (C) B6 mice were subjected to tibial fracture surgery and received either PBS or the clone 30H12 (mAb) as described in (A). In addition, on the second day following the fracture surgery, half of the animals that were administered with PBS or the clone 30H12 received no treatment and the other half received the AAV.COX-2 vector in the fracture sites. In addition, a group of animals that received only a control AAV vector in fracture sites was also included as a control. On days 4, 10, and 21, the fractured bones were examined by x-ray. On day 21, the fractured bones were harvested and analyzed by micro-computed tomography (μCT). Data are shown in (D) to (G). (D) Representative x-ray images on day 21 are shown (the contours indicate callus sizes). (E) The callus sizes were quantified by an imaging software. Data show the cumulative callus areas of five mice in each group. (F) The following parameters of new bone formation from the μCT analysis are shown: connectivity density (Conn. density), bone volume/volume of interest (BV/TV), bone mineral density (BMD), trabecular number (DT-Tb.N), trabecular thickness (DT-Tb.Th), and trabecular space (DT-Tb.sp). (G) Representative reconstructed three-dimensional images are shown. Where applicable, data are means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001; n = 3 to 5. Samiksha Wasnik et al. Sci Adv 2019;5:eaaw2108 Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).