Antibody titers against surface antigens of B Antibody titers against surface antigens of B. trehalosi in vaccinated and control groups of BHS. Serum antibody titers against B. trehalosi T10 surface antigens were determined by indirect ELISA. Three independent assays were performed using serum samples from all the animals. Antibody titers against surface antigens of B. trehalosi in vaccinated and control groups of BHS. Serum antibody titers against B. trehalosi T10 surface antigens were determined by indirect ELISA. Three independent assays were performed using serum samples from all the animals. Each data point represents the geometric mean titer of one animal in the respective group. The horizontal bar represents the geometric mean titer of the group at each time point. The vaccinated group had significantly higher mean titers than the control group (P ≤ 0.0001) following vaccination. Titers are shown as the natural logarithm (LN) of the reciprocal dilution. Open circles represent control animals; closed triangles indicate vaccinated animals. DOC, day of challenge; gp, group. Titers of <100 were considered 100 for the purpose of analysis. Renuka Subramaniam et al. Clin. Vaccine Immunol. 2011; doi:10.1128/CVI.05276-11