Taylor A. Doherty, MD, Rachel Baum, BS, Robert O

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Presentation transcript:

Group 2 innate lymphocytes (ILC2) are enriched in active eosinophilic esophagitis Taylor A. Doherty, MD, Rachel Baum, BS, Robert O. Newbury, MD, Tom Yang, BS, Ranjan Dohil, MD, Melissa Aquino, BS, Ashmi Doshi, MD, Hannah H. Walford, MD, Richard C. Kurten, PhD, David H. Broide, MBChB, Seema Aceves, MD, PhD Journal of Allergy and Clinical Immunology Volume 136, Issue 3, Pages 792-794.e3 (September 2015) DOI: 10.1016/j.jaci.2015.05.048 Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Identification of esophageal ILC2s. Single-cell suspensions from esophageal biopsies from a patient with active EoE (top) and a non-EoE control individual (bottom) were stained for CD45, lineage (CD3, CD14, CD16, CD19, CD20, CD56, TCRγδ, CD4, CD11b, CD235a, and FCeRI), and CRTH2 or isotype. ILC2s are identified as CD45+ low FSC lymphocytes that are lineage-negative and express CRTH2. FSC, Forward scatter; SSC, side scatter. Journal of Allergy and Clinical Immunology 2015 136, 792-794.e3DOI: (10.1016/j.jaci.2015.05.048) Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Increased ILC2s correlate with active EoE and tissue eosinophilia. A, ILC2s were quantified (% of live cells) in control non-EoE biopsy tissue as well as in patients with active EoE, inactive EoE, and PPI-REE (P = .005, 1-way ANOVA). B, %ILC2s of live cells correlated with mean (bottom, Pearson r = 0.7, P < .0001, 95% CI, 0.45-0.84) eosinophils. Journal of Allergy and Clinical Immunology 2015 136, 792-794.e3DOI: (10.1016/j.jaci.2015.05.048) Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E1 Collection of donor esophageal lymphoid aggregates. Lymphoid aggregates were identified as pale yellow discs (∼1 × 0.8 × 0.4 mm, white arrow) on the submucosal surface (left) after removal of Meissner's plexus and collected by sharp dissection (right). Journal of Allergy and Clinical Immunology 2015 136, 792-794.e3DOI: (10.1016/j.jaci.2015.05.048) Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E2 Human esophageal ILC2s expand in response to IL-33 and TSLP. Lymphoid aggregates from donor esophagus were processed into single-cell suspensions and cultured with IL-2 with or without IL-33 and TSLP for 1 week. A, Gating strategy for identification of ILC2s postculture. CD45+ cells were identified followed by gating of lymphocytes, lineage-negative, and CRTH2+ cells. B, Total number of ILC2s per well after culture. Results shown are 1 of 3 independent experiments. Mean ± SEM, t test. FSC, Forward scatter; SSC, side scatter. Journal of Allergy and Clinical Immunology 2015 136, 792-794.e3DOI: (10.1016/j.jaci.2015.05.048) Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions