In vivo localization of Iff2, Iff3 (Iff9), Iff5, Iff6, Iff8, and Dcw1 proteins. In vivo localization of Iff2, Iff3 (Iff9), Iff5, Iff6, Iff8, and Dcw1 proteins. (A) Fixed transformed cells were directly treated with anti-V5–Cy3–coupled antibody, and immunofluorescence was observed using a microscope. (B) Cells were permeabilized prior to addition of anti-V5–Cy3–coupled antibody. (C) Fixed cells were incubated in the presence of proteinase K for 2 h at 37°C prior to immunofluorescence detection. Anita Boisramé et al. Eukaryotic Cell 2011; doi:10.1128/EC.05044-11