Activities of layer 2/3 motor cortex cells during task performance.

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Activities of layer 2/3 motor cortex cells during task performance. Activities of layer 2/3 motor cortex cells during task performance. A, A representative example of a two-photon imaged field in the RFA. Depth from the cortical surface was 188 μm. Circles surround reconstructed cells. Parts of fluorescent traces of numbered, closed circles are shown in B. Cyan, magenta, yellow, and gray circles indicate pull cells, post-pull cells, other task-related cells, and non-task-related cells, respectively. B, Traces (270 s) of motion-corrected calcium transients of the numbered cells shown in A. The trace indicated as Lever shows the lever trajectory. Shaded boxes indicate successful trials. The traces indicated as Δx, Δy, and Δθ are motion-corrected displacements of X, Y, and rotation-angle shifts, respectively. Cells 1–4 were pull cells, cells 5–7 were post-pull cells, and cells 8–9 were non-task-related cells. C, The motion-corrected traces of cells 1, 5, and 9 are aligned with the start of all 30 successful lever pulls during the imaging session. Black thick lines indicate the mean traces. The lever trajectories in individual trials are overlaid in the bottom trace. Riichiro Hira et al. J. Neurosci. 2013;33:1377-1390 ©2013 by Society for Neuroscience