Paper review Miyeon Shin 21 Apr 2015

Reason for selection

Materials and Methods Rainbow trout n = 15 (Total n = 60) 1) Design of In Vivo study Conc. of spiked food :BDE 209 [940±14 ng/g w.w] Dosing volume : 1% of B.W/day Dosing duration : 5 months (5 days per week) Sample type : Blood, liver, intesti -nal tissue, carcass Exposure Gr. Control Gr.

Materials and Methods Used cell line : 1) Liver of rainbow trout 1) Design of In Vitro study Common carp Used cell line : 1) Liver of rainbow trout 2) common carp

Results and Discussion 1) In Vivo experiment

Results and Discussion 1) In Vivo experiment Steady state 3.5 month

Results and Discussion 1) In Vivo experiment Lipid normalized BDE 209 * * Lipid content was assumed.

Results and Discussion 1) In Vivo experiment $ * NonaBDEs $ OctaBDEs & HeptaBDEs $ * ECNI (electron captgure negative ion) & $ $ & & $ * * & $

Results and Discussion 1) In Vivo experiment ECNI (electron captgure negative ion)

Results and Discussion 1) In Vivo experiment Kierkegaard et al., (1999) ES&T Input dose 9.4 μg of BDE 209/kg body weight/day 무지개송어의 근육에서 BDE209의 debromination을 관찰, 시간에 따라 증가하는 것이 보임.

Conceptual model of potential metabolic transformation pathways for decaBDE in fish

Results and Discussion 1) In Vivo experiment Consist of Data on day 112 202 (19%), 201 (9%) 32% 26% 208 (31%) 94% 37% 68% Approx. 30%

Results and Discussion 1) In Vivo experiment Due to the size or higher serum protein binding affinities of BDE 209 209 208 207 206 . Diffusion Liver Tissue for Fish Place for biotransform. of chemicals Preferential accumul. of lighter congeners BDE 209 -> lower congeners

Results and Discussion 1) In Vivo experiment Log Kow increases with the degree of bromination Log Kow: BDE 209 (10) octaBDE (8.0 - 8.4) pentaBDE (6.5 - 7.0) tetraBDE (5.9 - 6.2) Easy to disposition in liver or serum Ref) Eljarrat et al., Brominated Flame Retardants- the handbook of env. chem.

Results and Discussion 1) In Vivo experiment Common carp Rainbow trout The previous study (2004) The present study (2006)

Results and Discussion 1) In Vivo experiment Both fish have the capability to debrom. BDE209, But differ in the extent and Probably the rate of debom. Common carp BDE 202 There’s no BDE209 in whole body of the carp -> Carp have an enhanced metabolic ability Ref) Stapleton et al., (2004), ES&T 38: 112-119 In vitro test

Ref) 1) Wikipedia, 2) www.lookfordiagnosis.com

Results and Discussion 1) In Vitro experiment Incubated for 1hr and 24hr At 25℃ BDE 209 15 pmole/mg of protein Control 실험내용 및 결과에 대해서도 언급할 것

Results and Discussion 1) In Vitro experiment Biotransformed 65% of BDE209 Biotransformed 22% of BDE209

Results and Discussion 1) In Vitro experiment Rainbow trout Common carp In vitro In vivo

Deiodinase(DI) enzymes DI contributes to breakdown of thyroid hormones. It releases iodine.