A dynamical model of the spindle position checkpoint

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A dynamical model of the spindle position checkpoint

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A dynamical model of the spindle position checkpoint Tem1, Bfa1 and Bub2 levels at the SPBs. (A) Box‐whisker plots representing the number of molecule distributions of SPB‐bound Tem1–GFP, Bfa1–GFP and Bub2–GFP in kar9Δ cells during anaphase. (B) Mean fluorescence intensities of Bfa1–3mCherry and Tem1–GFP at the SPBs during mitosis. (C) Box‐whisker plots of SPB‐bound Tem1–GFP number of molecules in bfa1Δ bub2Δ cells during early (2 μm <spindle length<4 μm) and late anaphase (spindle length>4 μm). (D) Mean fluorescence intensity of SPB‐bound Tem1–GFP in bfa1Δ bub2Δ cells during mitosis. In box‐whisker plots, upper and the lower boundaries of the boxes are the third and the first quartiles. The lines and circles in the boxes show the median and mean values, respectively. Whiskers indicate the tenth and the ninetieth percentiles. Mean fluorescence intensity line graphs are the average of ‘n’ cells monitored with 1‐min time resolution. In each cell, maximum signal of each protein is normalized to 1. The distances between the SPBs (pole‐to‐pole distances) are plotted as a reference for cell cycle progression. Source data is available for this figure in the Supplementary Information. IF THIS IMAGE HAS BEEN PROVIDED BY OR IS OWNED BY A THIRD PARTY, AS INDICATED IN THE CAPTION LINE, THEN FURTHER PERMISSION MAY BE NEEDED BEFORE ANY FURTHER USE. PLEASE CONTACT WILEY'S PERMISSIONS DEPARTMENT ON PERMISSIONS@WILEY.COM OR USE THE RIGHTSLINK SERVICE BY CLICKING ON THE 'REQUEST PERMISSIONS' LINK ACCOMPANYING THIS ARTICLE. WILEY OR AUTHOR OWNED IMAGES MAY BE USED FOR NON-COMMERCIAL PURPOSES, SUBJECT TO PROPER CITATION OF THE ARTICLE, AUTHOR, AND PUBLISHER. Mol Syst Biol, Volume: 8, Issue: 1, First published: 08 May 2012, DOI: (10.1038/msb.2012.15)