Main Phase Transitions in Supported Lipid Single-Bilayer

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Main Phase Transitions in Supported Lipid Single-Bilayer A. Charrier, F. Thibaudau  Biophysical Journal  Volume 89, Issue 2, Pages 1094-1101 (August 2005) DOI: 10.1529/biophysj.105.062463 Copyright © 2005 The Biophysical Society Terms and Conditions

Figure 1 AFM images (8×2.5μm) of the lipid bilayer formation in 10−2M NaCl. Between each image, 2.5μl of DMPC at 10mg/ml has been added and stirred in the solution. The vesicle fusion is self-limited after the first bilayer. Biophysical Journal 2005 89, 1094-1101DOI: (10.1529/biophysj.105.062463) Copyright © 2005 The Biophysical Society Terms and Conditions

Figure 2 AFM images (3×3μm) of transitions 1 and 2 of the DMPC supported single-bilayer at different temperatures. The darkest areas are holes in the bilayer. The sample is continuously heated under the AFM tip at a rate of 0.1°C/min. The temperature indicated in each image corresponds to the temperature in the middle of the image. Biophysical Journal 2005 89, 1094-1101DOI: (10.1529/biophysj.105.062463) Copyright © 2005 The Biophysical Society Terms and Conditions

Figure 3 Experimental curves of transition 1 (open triangles) and evolution of holes (solid triangles) at increasing and decreasing temperatures. Biophysical Journal 2005 89, 1094-1101DOI: (10.1529/biophysj.105.062463) Copyright © 2005 The Biophysical Society Terms and Conditions

Figure 4 Calculated curve of the percentage of gel phase versus temperature. In this calculation we consider a simple transition at constant surface and constant matter. Biophysical Journal 2005 89, 1094-1101DOI: (10.1529/biophysj.105.062463) Copyright © 2005 The Biophysical Society Terms and Conditions

Figure 5 Experimental and modeled curves of the two transitions. The experimental curves are the average of three different measurements. Biophysical Journal 2005 89, 1094-1101DOI: (10.1529/biophysj.105.062463) Copyright © 2005 The Biophysical Society Terms and Conditions