Functional analysis of PVY CP N- and C-terminal deletions in planta

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Fig. 6 Functional analysis of PVY CP N- and C-terminal deletions in planta. Functional analysis of PVY CP N- and C-terminal deletions in planta. The effect of deletion mutants ΔN50-CP, ΔC40-CP, and ΔC60-CP was evaluated by bombardment of N. clevelandii with respective PVY clones. (A) Local multiplication in bombarded leaves: Relative concentration of PVY RNA was measured in individual leaves 7 days postbombardment (dpb). Results are given as the frequency of leaves with detected RNA and as a comparison of viral RNA quantities measured in the bombarded leaves. Differences were statistically evaluated using one-way analysis of variance (ANOVA) with Bonferroni post hoc analysis. Means not sharing the same letter are significantly different (P < 0.05). Error bars represent SE. (B) Local multiplication in bombarded leaves: VLPs were detected by negative-stain TEM of samples from bombarded leaves. (C) Long-distance movement: Relative concentration of viral RNA was measured in upper nonbombarded leaves (table; 14 dpb). Only wild-type (WT) PVY caused typical disease symptoms in N. clevelandii plants (bottom; 22 dpb). (Photo credit: Ion Gutierrez-Aguirre, National Institute of Biology, Slovenia.)‏ Andreja Kežar et al. Sci Adv 2019;5:eaaw3808 Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).