Forensic Characterization of Semen: 1. VISUALIZATION

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Presentation transcript:

Forensic Characterization of Semen: 1. VISUALIZATION MANY BODY FLUIDS FLUORESCE WITH ALTERNATE LIGHTING SOURCES

Forensic Characterization of Semen: 2. PRESUMPTIVE TESTING ACID PHOSPHATASE ENZYME / FOUND IN LARGE CONCENTRATIONS IN SEMEN

Forensic Characterization of Semen: 3. CONFIRMATION A. MICROSCOPIC IDENTIFICATION OF SPERM B. DETECTION OF P30, A MALE PROSTATE PROTEIN. USEFULL FOR VASECTOMIZED MALES

Intact spermatozoa on a vaginal slide

Forensic Characterization of Saliva Detection of Amylase (breaks down starch in gel) Evidence commonly tested for the presence of saliva includes: Cigarette butts Envelope flaps Swabs taken from the body of sexual assault victims Bottles, cans, & straws Gel contains starch that is broken down (circles ) in the presence of Amylase enzyme

Nucleated epithelial cells from a cigarette butt

Microscopic detection of Hair shaft and root

Large piece of tissue from fingernail scrapings

Safety First—safeguards while handling biological evidence Wear gloves Keep contaminated surface away from face—protect those mucous membranes Properly dispose of gloves/wash hands